相关实验视频

Updated: Jun 7, 2025

Semi-automatic PD-L1 Characterization and Enumeration of Circulating Tumor Cells from Non-small Cell Lung Cancer Patients by Immunofluorescence

Semi-automatic PD-L1 Characterization and Enumeration of Circulating Tumor Cells from Non-small Cell Lung Cancer Patients by Immunofluorescence

Published on: August 14, 2019

肺状细胞癌中lncRNA TPT1-AS1的表达及其预后值

Lixin Yu¹, Zhenkui Zhang², Zhijian Wang²

¹Department of Thoracic Surgery, Beijing Chao-Yang Hospital, Capital Medical University, 5 Jingyuan Road, Shijingshan District, Beijing, 100020, China. lixinyu9632@163.com.

Discover oncology

|November 11, 2024

概括

长非编码RNATPT1-AS1,当过度表达时,可以通过向miR-4726-5p来抑制肺平细胞癌 (LUSC) 的进展. TPT1-AS1作为LUSC患者的潜在预后标志物.

科学领域:

在瘤学瘤学.
分子生物学分子生物学
遗传学遗传学是一个

背景情况:

肺状细胞癌 (LUSC) 的预后不佳,需要新的预后标志物.
长非编码RNAs (lncRNAs) 的异常表达与各种癌症有关.
一种lncRNA的TPT1-AS1在多种癌症类型中表达变化.

研究的目的:

为了调查TPT1-AS1在LUSC中的预后意义.
在LUSC.中探索TPT1-AS1和miR-4726-5p之间的关系.
评估TPT1-AS1在LUSC进展中的功能作用.

主要方法:

在使用RT-qPCR的115名LUSC患者中,对TPT1-AS1和miR-4726-5p的表达分析.
TPT1-AS1表达和临床病理特征之间的相关性分析.
双露西法酶记者测定证实了TPT1-AS1针对miR-4726-5p.p.的向.
细胞增殖,迁移和入侵测定 (CCK-8,Transwell) 来评估功能影响.

主要成果:

在LUSC组织中,TPT1-AS1表达的下调,而miR-4726-5p的上调.
较低的TPT1-AS1表达与较短的整体存活率相关,并将TPT1-AS1确定为独立的预后因素.
TPT1-AS1直接准miR-4726-5p,在瘤中观察到它们的表达水平之间存在逆相关性.
过度表达TPT1-AS1抑制了LUSC细胞的增殖,迁移和入侵.

结论:

在LUSC中,TPT1-AS1通过准miR-4726-5p作为瘤抑制剂.
TPT1-AS1有可能成为LUSC.的预后生物标志物.
调节TPT1-AS1表达可能为LUSC.提供治疗策略.

关键词:

肺状细胞癌是肺的状细胞癌.在MiR-4726-5p中使用.预测预后预测预测在 TPT1-AS1 的基础上.

更多相关视频

Development of Compendium for Esophageal Squamous Cell Carcinoma

Development of Compendium for Esophageal Squamous Cell Carcinoma

Published on: April 12, 2024

Exosomal miRNA Analysis in Non-small Cell Lung Cancer NSCLC Patients' Plasma Through qPCR: A Feasible Liquid Biopsy Tool

Exosomal miRNA Analysis in Non-small Cell Lung Cancer NSCLC Patients' Plasma Through qPCR: A Feasible Liquid Biopsy Tool

Published on: May 27, 2016

相关概念视频

lncRNA - Long Non-coding RNAs

lncRNA - Long Non-coding RNAs

In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...

Non-LTR Retrotransposons

Non-LTR Retrotransposons

As the name suggests, non-LTR retrotransposons lack the long terminal repeats characteristic of the LTR retrotransposons. Additionally, both LTR and non-LTR retrotransposons use distinct mechanisms of mobilization. Non-LTR retrotransposons are further divided into two classes - Long interspersed nuclear elements (LINEs) and short interspersed nuclear elements (SINEs), both of which occur abundantly in most mammals, including humans. Some of the active non-LTR retrotransposons in humans are L1...