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微卫星标记物和线粒体D-循环基于Gabrali牛的植物遗传和多样性分析

Farhad Ali¹, Syed Muhammad Suhail¹, Farhan Anwar Khan¹

¹The University of Agriculture, Peshawar, Pakistan.

Tropical animal health and production

|November 11, 2024

概括

巴基斯坦本土的加布拉利牛品种显示出大量的遗传多样性和与Bos indicus的共同祖先. 这项研究证实,这一重要的多用途品种没有直接面临遗传瓶的威胁.

科学领域:

动物遗传学动物遗传学
分子原生学分子原生学
畜牧生物多样性畜牧生物多样性

背景情况:

加布拉利 (Gabrali) 牛是巴基斯坦开伯帕赫顿克瓦 (Khyber Pakhtunkhwa) 的原产品,具有重要的经济意义.
尽管它有价值,但科学数据缺乏关于它的遗传多样性和遗传学关系.

研究的目的:

研究加布拉利牛品种的遗传多样性和遗传学状况.
为了解决关于这种多用途牲畜种群的科学数据的稀缺性.

主要方法:

从30头不相关的加布拉利牛的血液样本中提取了DNA.
微卫星位点和线粒体DNA的D环区域使用PCR被放大.
桑格测序用于D-循环安普利康,微卫星数据使用GeneAlEx®软件进行分析.

主要成果:

微卫星分析揭示了83个等位基因,观察到的异构性高 (0.58) 和预期的异构性高 (0.50),表明正常的等位基因分布和与哈迪-韦恩伯格平衡一致.
D-循环测序确定了8种单核型和30种单核酸多态 (SNP),其中AT含量高于GC含量 (44.1%),而AT含量高于55.9%).
遗传学分析表明,Gabrali和Bos indicus类型的单类,表明了来自国家牛品种的基因侵入.

结论:

加布拉利牛与Bos indicus有着共同的祖先.
该品种表现出大量的遗传多样性,目前没有面临遗传瓶.
这些发现强调了保护加布拉利牛品种的重要性.

关键词:

森林的指标 Bos indicus.这是一个D-循环.加布拉利牛牛遗传多样性遗传多样性微卫星标记器的使用

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