相关实验视频

Updated: May 11, 2026

Expression, Isolation, and Purification of Soluble and Insoluble Biotinylated Proteins for Nerve Tissue Regeneration

Expression, Isolation, and Purification of Soluble and Insoluble Biotinylated Proteins for Nerve Tissue Regeneration

Published on: January 22, 2014

通过应用响应表面方法,通过孤立的Bacillus菌株增强维生素B₁₂的产生

Rania M M Abdel-Baki¹, Marwa N Ahmed², Olfat S Barakat¹

¹Department of Microbiology, Faculty of Agriculture, Cairo University, El-Gamaa Street, Giza, 12613, Egypt.

BMC biotechnology

|November 11, 2024

概括

这项研究确定了Peribacillus acanthi作为一种通过微生物发酵产生维生素B12的新型,高产的生产者. 优化条件和使用糖蜜作为碳来源大大降低了生产成本.

科学领域:

微生物学微生物学
生物技术是生物技术.
生物化学工程生物化学工程

背景情况:

维生素B12是必不可少的,但化学合成具有挑战性.
微生物发酵提供了一个替代的生产途径.
糖蜜为维生素B12生产提供了具有成本效益的碳来源.

研究的目的:

选微生物分离物以检测高维生素B12的产生.
为了识别和表征新的维生素B12生产菌株.
为了优化维生素B12的生产,使用具有成本效益的基质.

主要方法:

选87种酵母,菌体和细菌分离物.
使用MALDI-TOF和分子方法识别高产分离物.
优化发酵条件 (糖类型,注射剂大小,反应表面方法).

主要成果:

鉴定了Bacillus subtilis,Bacillus sp. 和Peribacillus acanthi的菌株. 这些菌株是:
皮里巴西勒斯阿坎蒂MZ01证明了高的维生素B12生产力.
优化条件和糖蜜中等显著提高了产量.

结论:

帕里巴西勒斯 (Peribacillus acanthi) 是一种新发现的,高产量的维生素B12生产物.
优化的发酵条件提高了效率.
使用糖蜜可以显著降低维生素B12的生产成本.

关键词:

周围细菌 (peribacillus acanthi) 是一个亚诺科巴胺是什么意思?甲基可巴胺是什么甲基可巴胺是什么糖蜜糖是什么意思糖蜜糖是什么意思标签缅甸设计维生素B12是一种维生素.

更多相关视频

Using Synthetic Biology to Engineer Living Cells That Interface with Programmable Materials

Using Synthetic Biology to Engineer Living Cells That Interface with Programmable Materials

Published on: March 9, 2017

Expression of Exogenous Antigens in the Mycobacterium bovis BCG Vaccine via Non-genetic Surface Decoration with the Avidin-biotin System

Expression of Exogenous Antigens in the Mycobacterium bovis BCG Vaccine via Non-genetic Surface Decoration with the Avidin-biotin System

Published on: January 31, 2018

相关概念视频

Bioreactor Controls-III

Bioreactor Controls-III

Strain improvement is a foundational strategy in industrial microbiology aimed at maximizing microbial productivity, particularly because natural isolates typically yield commercially valuable products in very low concentrations. Although optimizing the culture medium and environmental conditions can improve yields, these adjustments are inherently limited by the organism’s genetic potential. As a result, the focus shifts toward genetic modifications to enhance biosynthetic capacity. The...

Production of Biopesticides

Production of Biopesticides

Biopesticides offer a sustainable alternative to chemical pesticides, utilizing microbial agents to control agricultural pests. Bacillus thuringiensis (Bt) is a widely employed bacterium known for its potent insecticidal activity. Bt biopesticides are favored for their specificity to insect pests, minimal environmental impact, and natural degradability.Mechanism of Bt Toxin Action Bt produces insecticidal crystal (Cry) proteins during its sporulation phase. These proteins form parasporal...