益生菌衍生的细胞外囊泡通过调节肠道微生物群和AHR激活来缓解AFB1诱导的肠道损伤
Jinyan Li1,2,3, Mengdie Shi1,2,3, Yubo Wang1,2,3
1College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu Province, 210095, China.
Journal of nanobiotechnology
|November 11, 2024
概括
乳杆菌类细胞外囊泡 (LA.EVs) 通过调节肠道微生物群和激活AHR/IL-22通路来保护肠道免受阿弗拉托xin B1 (AFB1) 损伤. 这促进了肠道屏障的修复,并减少了炎症.
科学领域:
- 微生物学 微生物学
- 毒理学 毒理学 毒理学
- 胃肠病学 胃肠病学
背景情况:
- 非洲毒素B1 (AFB1) 是一种普遍存在的真菌毒素,可引起显著的肠道损伤.
- 乳杆菌菌 (LA) 和其细胞外囊泡 (EVs) 涉及肠道健康,但它们对AFB1的保护机制尚不清楚.
研究的目的:
- 调查乳酸菌氨酸-QC1H衍生的EVs (LA.EVs) 对AFB1诱导的肠损伤的保护作用和潜在机制.
主要方法:
- 隔离Lactobacillus amylovorus-QC1H菌株并提取其EVs.
- 评估LA.EV对小鼠AFB1诱导的肠损伤标志物的影响.
- 16S rRNA分析以评估肠道微生物群调节.
- 研究基碳化合物受体 (AHR) /介质素-22 (IL-22) 信号传递的作用.
主要成果:
- 通过减少促炎性细胞因子和肠道透性,LA.EVs显著缓解了AFB1诱导的肠道损伤.
- LA.EVs恢复了紧结蛋白的表达,并调节了AFB1诱导的肠道失调.
- LA.EVs增加了肠道的英多尔-3-酸 (IAA),并激活了AHR/IL-22信号,这对它们的保护作用至关重要.
结论:
- 在小鼠中,LA.EVs显示出显著的保护作用,防止AFB1诱导的肠损伤.
- 机制包括肠道微生物群调节,激活AHR/IL-22通路,减少炎症和增强肠道屏障修复.
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