不同的中性粒细胞效应因子功能,以响应不同的孤立的Leishmania aethiopica
E Adem1, E Cruz Cervera2, E Yizengaw3,4,5
1University of Greenwich, Medway Campus, Gillingham, UK.
Parasites & vectors
|November 11, 2024
概括
莱什马尼亚埃塞俄比亚寄生虫对中性粒细胞的功能有不同的影响,包括结合,内化,亡和ROS产生. 与临床隔离物相比,长期培养的寄生虫显示出明显的效应.
科学领域:
- 免疫学 免疫学 免疫学
- 寄生虫学的寄生虫学
- 细胞生物学 细胞生物学
背景情况:
- 埃塞俄比亚的皮肤莱什曼病是由莱什曼病 (L.) aethiopica.ca引起的.
- 主体免疫反应在L. aethiopica临床表现中的作用尚不清楚.
- 中性粒细胞是对沙咬伤的早期反应者,这使得它们对初始感染控制至关重要.
研究的目的:
- 调查L. aethiopica分离物和中性粒细胞之间的相互作用.
- 为了在体外评估中性粒细胞效应因子对L. aethiopica感染的功能.
- 为了确定寄生虫菌株是否影响中性粒细胞的反应.
主要方法:
- 在体外感染模型使用中性粒细胞和L. aethiopica分离物 (3临床,1种植).
- 流细胞测量以评估寄生虫协会和中性粒细胞种群 (CD15高/低).
- 孔焦显微镜用于寄生虫内化,亡试验和ROS生产测量.
主要成果:
- 所有与中性粒细胞相关的L. aethiopica分离物;临床分离物显示较高的关联.
- 对所有隔离品观察到中性粒细胞对寄生虫的内部化.
- L. aethiopica诱导了中性粒细胞亡和上调的ROS产量,随着时间的推移,分离物和中性粒细胞群之间的变化.
结论:
- 不同的L. aethiopica分离物对中性粒细胞效应器功能产生不同的影响.
- 与临床隔离物相比,长期培养的寄生虫对中性粒细胞反应产生了明显的影响.
- 中性粒细胞的反应,包括ROS的产生,在感染后的CD15高和CD15低种群之间存在差异.
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