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与犬老化项目中缺失的生物样本相关的因素

Sydney N Holland¹, Amanda K Tinkle¹, Jena N Prescott¹

¹Department of Small Animal Clinical Sciences, Texas A&M College of Veterinary Medicine & Biomedical Sciences, College Station, Texas, USA.

Veterinary medicine and science

|November 12, 2024

概括

在狗衰老项目 (DAP) 精密队列中发现了缺失的生物样本样本,特别是用于流细胞计的EDTA样本. 更长的接收时间与更大的样本损失相关,这表明需要进行程序调整.

科学领域:

兽医科学兽医科学兽医科学
基因组学就是基因组学.
纵向研究纵向研究

背景情况:

狗老龄化项目 (DAP) 是一项关键的纵向研究,研究了美国狗的老龄化.
缺少的数据,特别是生物标本样本,威胁到DAP发现的有效性和统计能力.
积极识别和减轻样本损失对于研究完整性至关重要.

研究的目的:

评估犬老化项目的精密队伍中缺少的生物标本样本.
确定导致样本缺失的因素.
建议策略,以减轻未来的样本损失在犬老化研究.

主要方法:

在2021年8月至10月期间收集的90个样本套件中评估了个别组件的缺失.
对各种样本类型 (例如EDTA,PBMCs,血,血清,便) 的评估失踪率.
分析了缺失与时间到收到和分数序列等因素之间的关系.

主要成果:

在EDTA样本 (18.9%),外周血液单核细胞 (PBMC) (13.3%) 和DNA (6.7%) 中观察到高缺失率.
便和尿液样本显示分别有5.6%和2.2%的缺失.
增加到收到的时间和连续的aliquot创建与更高比例的缺失样本有关.

结论:

建议修订样本采集说明,以解决经常缺失的样本类型.
需要对特定的采集程序进行进一步的调查,以确定样本产量损失的原因.
实施程序调整可以提高犬老化研究的数据完整性.

关键词:

生物标本生物标本血液血液血液血液血液狗类犬类动物狗类犬类失踪失踪失踪失踪失踪

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