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产前和产后免疫挑战会影响断奶猪的肌肉生长和新陈代谢

Thomas W Dobbins¹, Luke K Fuerniss¹, Manuel S Hernandez¹

¹Department of Animal and Food Sciences, Texas Tech University, Lubbock, TX 79409, USA.

Journal of animal science

|November 12, 2024

概括

孕期和产后LPS挑战改变小猪的肌肉发育和新陈代谢. 这项研究揭示了暴露于LPS的后代的长脊 (LD) 肌肉纤维大小和代谢物概况的显著变化.

科学领域:

动物科学动物科学
免疫学免疫学免疫学
代谢学代谢学代谢学

背景情况:

胎内环境极大地影响胎儿和产后发育.
妊娠期间的免疫刺激可以对后代生理学产生持久的影响.

研究的目的:

研究子宫内脂多聚糖 (LPS) 暴露和产后LPS挑战对猪长背 (LD) 的代谢特征和肌肉超结构的影响.

主要方法:

孕母猪接受LPS或盐水,然后在断奶后代中接受LPS挑战.
使用质谱学和生物信息学工具分析了LD肌肉进行形态测量,超结构和代谢概况.
统计分析包括单变量,多变量和混合程序.

主要成果:

暴露于LPS的后代在肌重链 (MHC) I型和IIB/X型肌肉纤维的平均直径和横截面积增加.
代谢分析揭示了14种不同表达的代谢物和10种倾向于在暴露于LPS的猪的LD中不同.
在总核或特定的肌性调节因子 (MYF5,PAX7) 阳性核中没有发现显著差异.

结论:

在母猪中通过LPS进行子宫内免疫刺激,加上产后LPS挑战,显著改变了断奶猪的代谢特征和肌肉超结构.
这些发现强调了早期免疫挑战对肌肉发育和代谢途径的长期影响.

关键词:

胎儿编程胎儿编程是什么?免疫挑战免疫挑战脂多糖类糖化物 (Lipopolysaccharide) 是一种脂多糖类糖.代谢生物组的代谢生物组这是骨肌肉的骨架肌肉.

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