囊胺的免疫调节作用及其作为结核病宿主导治疗的潜在用途
Saeid Najafi-Fard1, Chiara Farroni1, Linda Petrone1
1Translational Research Unit, National Institute for Infectious Diseases Lazzaro Spallanzani-Istituto di Ricovero e Cura a Carattere Scientifico (IRCCS), Rome, Italy.
Frontiers in immunology
|November 12, 2024
概括
囊胺是一种潜在的结核病和COVID-19治疗方法,通过减少炎症Th1和Tc1细胞来调节免疫反应. 较低剂量保留这种效果而不会影响细胞活力,这表明它可以作为抗菌治疗的辅助剂.
科学领域:
- 免疫学 免疫学 免疫学
- 药理学 药理学是指药理学的学科.
背景情况:
- 囊胺是一种已批准用于治疗囊病的药物.
- 它正在被研究为对 Mycobacterium 结核病 (Mtb) 和 SARS-CoV-2 的宿主导疗法.
- 囊胺的免疫调节作用需要进一步研究.
研究的目的:
- 评估囊胺对人类外周血液单核细胞 (PBMCs) 的体外免疫调节作用.
- 评估囊胺对通过纯化蛋白衍生物 (PPD) 和葡萄球菌肠毒素B (SEB) 刺激的免疫反应的影响.
主要方法:
- 来自结核感染 (TBI),结核病 (TB) 和健康对照 (HC) 个体的PBMCs被使用PPD或SEB刺激.
- 细胞在6小时和24小时内接受了不同度的囊胺 (50μM400μM) 治疗.
- 分析了T助手1 (Th1) 和T细胞毒性1 (Tc1) 细胞因子的产生,亡和亡.
主要成果:
- 半胺 (400μM) 在结核病和TBI受试者中显著降低了PPD特异性的Th1反应.
- 400微米和200微米的囊胺都显著降低了SEB诱导的Th1和Tc1反应.
- 高剂量的囊胺诱导淋巴细胞亡和亡,以剂量和时间依赖的方式.
结论:
- 高剂量的囊胺在体外降低了Th1和Tc1细胞的百分比,并诱导了亡/亡.
- 较低的囊胺剂量表现出免疫调节作用,而不会损害细胞活力.
- 囊胺可以作为一种辅助疗法,以减少结核病和COVID-19等传染病的炎症.
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