相关实验视频

Updated: Jun 7, 2025

Stem Cell-Derived Viral Ag-Specific T Lymphocytes Suppress HBV Replication in Mice

Stem Cell-Derived Viral Ag-Specific T Lymphocytes Suppress HBV Replication in Mice

Published on: September 25, 2019

抗原引导的切换策略从细胞核到干扰素:避免病毒学突破并改善功能治疗

Da Huang^1,2, Zhize Yuan¹, Di Wu^1,2

¹Department and Institute of Infectious Disease, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, China.

Journal of medical virology

|November 12, 2024

概括

高基线水平的乙型肝炎核心相关抗原 (HBcrAg) 和HBsAg预测慢性乙型肝炎 (CHB) 患者的病毒学突破 (VBT),转换为基化干扰素α (Peg-IFN-α). 这些标志物表明免疫反应不佳,并阻碍功能治愈.

科学领域:

肝病学肝病学是一种肝病学.
病毒学病毒学
免疫学免疫学免疫学

背景情况:

慢性乙型肝炎 (CHB) 管理涉及顺序治疗,但从核胺类类似物 (NA) 转换为基化干扰素α (Peg-IFN-α) 后,预测病毒学突破 (VBT) 的因素仍然不清楚.
了解这些因素对于优化治疗策略和改善HBsAg损失等结果至关重要.

研究的目的:

在接受顺序Peg-IFN-α治疗方案的CHB患者中确定VBT的预测因子.
研究基线病毒标记物,免疫反应和治疗结果之间的关系,包括HBsAg损失和功能治愈.

主要方法:

对接受48周Peg-IFN-α加NA治疗的80名CHB患者的回顾性分析,随后接受48周Peg-IFN-α单疗法.
动态监测HBVDNA,HBsAg,HBcrAg,HBeAg,肝脏内ccDNA和免疫生物标志物 (CD8+ T细胞,Tfh,B细胞).

主要成果:

12名患者 (15.0%) 经历了VBT,治疗后HBsAg损失率显著降低 (0%vs35.3%).
基线升高的HBcrAg (≥5 log10 U/mL) 和HBsAg (≥100 IU/mL) 与最高的VBT风险和未能达到HBsAg清除有关.
患有高HBcrAg水平的患者具有更高的肝脏内ccDNA. 与风险较低的组相比,高HBcrAg/HBsAg组表现出免疫反应减弱 (CD8+ T细胞,Tfh,B细胞).

结论:

基线HBcrAg和HBsAg水平是VBT的显著预测指标,以及在CHB中对顺序Peg-IFN-α治疗的反应不佳.
监测这些病毒标志物可以帮助识别患有VBT风险的患者,并指导改善功能治愈率的策略.
低于最佳的免疫反应与高的HBcrAg和HBsAg水平相关,突出显示了病毒载量和宿主免疫力在治疗结果中的相互作用.

关键词:

在NA-抑制的CHB中.佩格-IFN-ɑ 的意思功能性治疗治疗治疗免疫反应的免疫反应.病毒学突破病毒学突破

更多相关视频

Dissecting Innate Immune Signaling in Viral Evasion of Cytokine Production

Dissecting Innate Immune Signaling in Viral Evasion of Cytokine Production

Published on: March 2, 2014

Identifying Inhibitors of the HBx-DDB1 Interaction Using a Split Luciferase Assay System

Identifying Inhibitors of the HBx-DDB1 Interaction Using a Split Luciferase Assay System

Published on: December 21, 2019

相关概念视频

Viruses with RNA Genomes

Viruses with RNA Genomes

RNA viruses are categorized into positive-strand, negative-strand, or double-stranded groups based on their genomic structure and replication mechanisms. This classification dictates how they exploit host cellular machinery for protein synthesis and replication. Some RNA viruses also utilize reverse transcription as part of their life cycle, further diversifying their replication strategies.Positive-Strand RNA VirusesPositive-strand RNA viruses have genomes that function directly as messenger...

Retrovirus Life Cycles

Retrovirus Life Cycles

Retroviruses have a single-stranded RNA genome that undergoes a special form of replication. Once the retrovirus has entered the host cell, an enzyme called reverse transcriptase synthesizes double-stranded DNA from the retroviral RNA genome. This DNA copy of the genome is then integrated into the host’s genome inside the nucleus via an enzyme called integrase. Consequently, the retroviral genome is transcribed into RNA whenever the host’s genome is transcribed, allowing the...

Immune Response Against Viral Pathogens

Immune Response Against Viral Pathogens

The immune system's response to viral infections is a complex and coordinated process involving natural killer (NK) cells, T cell-mediated responses, and antibody-mediated responses.
NK Cells
NK cells are a crucial part of our innate immune system, acting as the first line of defense against viral infections. These cells can recognize and kill infected cells without prior exposure to the virus, effectively slowing down the spread of infection. Additionally, NK cells produce proinflammatory...