抗微生物SAAP-148-从具有广泛抗菌活性的可光交联聚合物的功能化水凝
Muhammad Atif1, Gizem Babuççu2, Martijn Riool2,3
1Macromolecular Chemistry, Department of Chemistry and Biology, University of Siegen, Adolf-Reichwein-Strasse 2, 57076, Siegen, Germany.
Macromolecular rapid communications
|November 12, 2024
概括
抗微生物 (AMP) 被固定在可光交叉链接的聚合物上,以创建有效的抗微生物伤口. 这种方法确保了对抗药性细菌的持续,非泄漏活性,为传统抗生素提供了有希望的替代品.
科学领域:
- 生物材料科学 生物材料科学
- 聚合物化学 聚合物化学
- 传染病研究 传染病研究
背景情况:
- 抗微生物 (AMP) 显示出治疗皮肤伤口感染的潜力,但其生物半衰期很短.
- 对AMPs在基质上的共价固定提供了长期抗微生物功能的解决方案.
- 开发先进的伤口包裹对于打击抗生素耐药性至关重要.
研究的目的:
- 合成和描述一种新型的可光交联聚合物,该聚合物与抗微生物SAAP-148.8功能化.
- 评估SAAP-148修饰聚合物及其水凝形式的抗菌疗效和细胞毒性.
- 评估在生物环境中固定AMP的长期稳定性和浸出概况.
主要方法:
- 使用自由基聚合来合成聚 ((HEAAm-co-BPAAm-co-PFPA-co-EH3A) 联合聚合物.
- 该共聚合物通过其氨基组与SAAP-148进行了修改,随后进行了UV光交联,以形成水凝网络.
- 对常见的伤口病原体 (大肠杆菌,金黄色杆菌,P. aeruginosa,A. baumannii) 进行了抗菌活性测试,包括多药耐药菌株.
- 使用人类肺纤维细胞质来评估细胞毒性.
- 浸出研究是在稀释的人类血和酸盐缓冲盐水 (PBS) 中进行的.
主要成果:
- 这种SAAP-148修饰的共聚物对人类肺纤维细胞无细胞毒性.
- 修改的聚合物溶液和光交联的水凝都显示出针对测试的细菌菌株的广泛抗菌活性.
- 联粘合物防止SAAP-148的浸出,在人体血中保持抗菌活性至少48小时,在PBS中保持14天.
- 水凝配方显示了持续的抗微生物疗效,特别是在人体血中.
结论:
- 具有SAAP-148功能的可光交联聚合物水凝是先进的抗菌伤口带的有希望的候选者.
- 这种材料为持续的抗微生物活性提供了一个稳定的,不泄漏的平台,解决了免费AMP的局限性.
- 开发的伤口带为传统抗生素提供了可行的替代方案,用于管理复杂的皮肤伤口感染.
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