来自OLP损伤的HSP90复合物通过激活树突细胞诱导T细胞极化
Lei Pan1,2,3,4,5, Junjun Chen2,3,4,5,6, Yirao Lai2,3,4,5,6
1Department of Second Dental Center, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
Oral diseases
|November 12, 2024
概括
口腔平坦 (OLP) 病变中的热冲击蛋白90 (HSP90) 复合体通过TLR9激活树突细胞 (DCs),促进Th17免疫力. 这一发现揭示了OLP的病原性.
科学领域:
- 免疫学 免疫学 免疫学
- 口腔病理学 口腔病理学
背景情况:
- 口腔平坦 (OLP) 是一种具有复杂免疫机制的炎症状况.
- 热冲击蛋白90 (HSP90) 涉及各种细胞应激反应和免疫调节.
研究的目的:
- 确定来自OLP病变的HSP90复合体在树突细胞 (DC) 激活中的作用.
- 为了研究OLP衍生的HSP90对原始T细胞极化的影响.
主要方法:
- 在OLP和健康对照中分析HSP90表达,使用单细胞RNA测序,IHC,qRT-PCR和免疫阻塞.
- 从OLP粘膜中提取的HSP90复合物作为DC激动剂.
- 通过流细胞计和共同培养实验评估的DC激活 (IFN-α,MHC表达) 和T细胞两极化 (细胞因子分析).
主要成果:
- 在OLP自身膜中观察到显著升高的HSP90水平,与淋巴细胞透相关.
- 由OLP衍生的HSP90复合体通过TLR9激活直流体,增强IFN-α分泌和MHC II表达.
- 用OLP HSP90复合体预刺激的DCs促进了Th17细胞的更高比例.
结论:
- 来自OLP病变的HSP90复合体通过TLR9/IFN-α通路激活DC.
- 这种激活促进了原始T细胞向Th17介导免疫的两极分化,有助于OLP的致病性.
相关概念视频
T Cell Types and Functions
954
When T cells with CD4 markers are activated, they give rise to two types of effector cells: helper T cells and regulatory T cells. Meanwhile, T cells with CD8 markers differentiate into effector cytotoxic T cells. The differentiation of CD4 T cells into helper T cell subsets, such as Th1, Th2, and Th17 cells, is dependent on the antigen type, antigen-presenting cell, and regulatory cytokines.
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...
954
T Cell Activation and Clonal Selection
671
T cells are integral to our adaptive immune system, recognizing and effectively responding to foreign antigens. T cell activation and clonal selection are pivotal in orchestrating this immune response. This article elucidates these mechanisms, detailing the roles of cluster of differentiation (CD) markers, major histocompatibility complex (MHC) molecules, costimulatory signals, and the process of clonal selection.
Naive T cells that have not yet encountered an antigen express two primary CD...
Naive T cells that have not yet encountered an antigen express two primary CD...
671


