从干细胞球体中产生细胞外囊的高产量生物生产,通过千万流体旋转转运输
Elliot Thouvenot1, Laura Charnay1, Noa Burshtein2
1Laboratoire Physique des Cellules et Cancer, PCC, CNRS UMR168, Institut Curie, Sorbonne Université, PSL Research University, Paris, 75005, France.
Advanced materials (Deerfield Beach, Fla.)
|November 12, 2024
概括
一种新型的毫流体芯片使生物活性细胞外囊泡 (EVs) 从最小的细胞材料中产生高产量. 这种方法利用受控的粘性应力来增强EV释放,提供了一个有前途的治疗性生产策略.
科学领域:
- 生物技术是生物技术.
- 再生医学是一种再生医学.
- 微流体学 微流体学
背景情况:
- 细胞外囊泡 (EVs) 对癌症和退行性疾病具有治疗潜力.
- 目前的方法缺乏高产量生产和有效使用细胞材料.
- 可扩展和具有成本效益的EV生产对于临床转换至关重要.
研究的目的:
- 开发一种用于高产量的细胞外囊泡 (EV) 生产的毫流体装置.
- 为了研究受控粘性应力对EV释放的影响.
- 用这种方法生产的电动汽车的生物活性和载荷的特征.
主要方法:
- 一个毫流体交叉槽芯片被设计用于产生受控的和粘性应力.
- 人类介质干细胞 (hMSC) 球体在芯片内培养和加工.
- 通过调整流量 (雷诺德数) 来调节电动汽车释放.
- 分析了EV的货物 (蛋白质组学,细胞计) 和生物活性 (血管生成,伤口愈合分析).
主要成果:
- 毫流体芯片在雷诺兹数≤400时实现了高产率的EV释放 (高达3万个EV/细胞).
- 控制的粘性应激诱导了EV的产生,而不会损害细胞完整性.
- 确定了一种控制EV分泌的压力依赖机制.
- 与单层衍生的EV相比,3D球形产生的EV表现出明显的分子特征和增强的亲血管性/伤口愈合能力.
结论:
- 毫流体交叉槽芯片为生产生物活性电动汽车提供了可扩展的方法.
- 控制的机械应力是提高电动汽车生产和功能的关键因素.
- 三维细胞微环境显著影响EV特征和治疗潜力.
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