细胞内ATP度是细菌细胞命运的关键调节者
Bo Li1, Xiao Chen1, Jin-Yu Yang2
1Biomedical Pioneering Innovation Center (BIOPIC), Beijing Advanced Innovation Center for Genomics (ICG), School of Life Sciences, Peking University, Beijing, China.
Journal of bacteriology
|November 12, 2024
概括
处于休眠状态的细菌,如可生存但不可培养 (VBNC) 和持久细胞,具有较低的细胞内ATP水平. 研究人员确定了一个ATP值来区分VBNC细胞,并通过增加ATP来恢复它们,为细菌控制提供了新的策略.
科学领域:
- 微生物学 微生物学
- 细胞生物学 细胞生物学
- 生物化学 生化学
背景情况:
- 细菌进入休眠状态,如可生存但不可培养 (VBNC) 和持久细胞,以在恶劣的条件下生存,其特点是代谢活动减少.
- 这些休眠状态对于持续的细菌感染至关重要,并且由于检测和治疗的困难,它们对根除构成挑战.
研究的目的:
- 研究细胞内腺三酸盐 (ATP) 度在细菌休眠中的作用.
- 根据ATP水平确定区分可培养和休眠细菌细胞的方法.
- 探索恢复休眠细菌细胞的策略.
主要方法:
- 使用敏感的ATP生物传感器 (QUEEN-7μ) 来测量单个细菌细胞中的细胞内ATP度.
- 使用光激活细胞分类 (FACS) 将VBNC细胞与可培养细胞分离,使用定义的ATP值.
- 研究了细胞内ATP水平对蛋白质沉和VBNC细胞形成的影响.
- 通过使用蛋白质素 (PR) 和绿光提高细胞内ATP,证明了VBNC细胞的复苏.
主要成果:
- 与可培养和抗生素敏感细胞相比,VBNC和持久细胞的细胞内ATP度明显较低,持久细胞和敏感细胞之间有一些重叠.
- 通过FACS建立了12.5μM的细胞内ATP度值,以区分VBNC和可培养细胞.
- 在关键的生物过程中蛋白质沉被证实可以促进VBNC细胞的形成.
- 通过增加它们的细胞内ATP水平,成功地实现了VBNC细胞的复苏.
结论:
- 细胞内ATP度是细菌细胞命运的关键调节者,影响过渡到休眠状态和维持休眠状态.
- 细菌休眠,VBNC和持续细胞形成与降低的ATP水平和相关的蛋白质沉密切相关.
- 调节细胞内ATP水平为控制细菌感染和恢复休眠细胞提供了一个有前途的治疗策略.
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