相关实验视频
Updated: Jun 7, 2025

06:24
Establishing 3D Endometrial Organoids from the Mouse Uterus
Published on: January 6, 2023
6.0K
含有WD重复蛋白-61调节子宫内膜上皮细胞粘附,表明它在受体性方面发挥着重要作用
Poppy Downing1,2, Madeleine Howe1,2, Michaela Sacco1,2
1Department of Obstetrics, Gynecology and Newborn Health, University of Melbourne, Parkville, Melbourne, Australia.
Molecular human reproduction
|November 12, 2024
概括
含WD重复蛋白-61 (WDR61) 对于子宫内膜接受性至关重要,促进胚胎植入. 减少WDR61会损害细胞粘附和增殖,强调其在生育方面的作用.
科学领域:
- 生殖生物学 生殖生物学
- 分子内分泌学分子内分泌学
- 细胞和分子生理学的细胞和分子生理学.
背景情况:
- 子宫内膜受容性对于成功的胚胎植入至关重要.
- 子宫内膜经历周期性变化,为胚胎囊附着做好准备.
- 调节子宫内膜受体性的机制尚未完全理解.
研究的目的:
- 研究WD重复含有蛋白-61 (WDR61) 在人类子宫内膜受体性中的作用.
- 为了确定WDR61在子宫内膜中的定位和表达模式.
- 阐明WDR61对子宫内膜上皮细胞粘附和增殖的功能影响.
主要方法:
- 免疫组织化学测定WDR61的定位和表达.
- RNA干扰 (siRNA) 在初级子宫内膜上皮细胞和石川细胞中敲击WDR61.
- 对胚胎囊球状粘附于WDR61敲击细胞的评估.
- 定量PCR (qPCR) 用于分析基因表达变化.
- 染色体免疫沉测序 (ChIP-seq) 用于识别WDR61的目标基因.
主要成果:
- WDR61定位在子宫内膜上皮细胞和脑膜细胞的细胞核和细胞质中.
- 与繁殖阶段相比,WDR61表达在受体中分泌阶段显著更高.
- 在石川细胞中,WDR61 knockdown 阻断了芽细胞球状粘附,降低了细胞增殖和粘附能力.
- 通过WDR61敲击,关键受体性基因 (HOXD10,MMP2,CD44) 的表达减少.
- ChIP-seq确定了2022个直接的WDR61点基因,这些基因参与焦点粘附,信号传递和上皮层-介质细胞过渡.
结论:
- WDR61在促进子宫内膜受体性方面发挥着至关重要的作用.
- WDR61调节子宫内膜上皮细胞的焦点粘附,增殖和上皮-介质细胞过渡.
- WDR61是改善生育能力和治疗与子宫内膜受体性降低相关的不孕症的潜在治疗标.
相关概念视频
Adherens Junctions
4.7K
Strong contact points between adjacent cells anchor them to each other, forming tissues. Such anchoring junctions are of two types – adherens junctions and desmosomes. Adherens junctions are abundant in tissues such as epithelium and endothelium, forming a continuous zone of adhesion called the adhesion belt. In other tissues, such as heart muscle, they appear as clusters, linking the cells to produce coordinated heart muscle contraction.
Adherens Junctions are Dynamic
Adherens Junctions are Dynamic
4.7K
Role of Ephrin-Eph Signalling in Intestinal Stem Cell Renewal
2.2K
Erythropoietin-producing hepatocellular carcinoma receptor (Eph) and its ligand, Eph receptor-interacting protein (Ephrin) were first discovered in the human carcinoma cell line, hence the name. Ephrin-Eph interaction guides cells to reach their appropriate location in adult tissues. They also play an essential role in the immune system by helping in immune cell migration, adhesion, and activation. Based on their structure and function, Eph is divided into two classes — EphA and EphB.
2.2K
Canonical Wnt Signaling Pathway
8.7K
The gene encoding the main signaling molecules of the Wnt signaling pathways (the Wnt proteins) was discovered almost four decades ago by Nüsslein-Volhard and Wieschaus. They identified and originally named the gene "wingless" (wg) after a phenotype discovered during their landmark genetic screen in Drosophila for body pattern defects. At around the same time, another researcher named Harold Varmus found that a murine tumor virus activates the mammalian wg homolog, Int-1, which...
8.7K
Anchoring Junctions
3.6K
Anchoring junctions are multiprotein complexes that help cells connect to other cells and the extracellular matrix. Anchoring junctions are present on the lateral and basal surfaces of cells, providing strong and flexible connections. Focal adhesions are often formed due to cell interactions with the ECM substrata, which initiate signal transduction via kinase cascades and other mechanisms. Together, they provide stability and tissue integrity. There are three types of anchoring junctions:...
3.6K
Non-Canonical Wnt Signaling Pathways
7.2K
Wnt is a zygotic effect gene that is expressed during very early embryonic development. It regulates various processes in animals starting from early development through the adult stage, such as organogenesis in the embryo and maintenance of neuronal and blood stem cells. Wnt proteins can induce a wide variety of intracellular pathways depending upon the specific abilities of different Wnt ligands to form a complex with shared and cognate receptors in the presence of different co-receptors. The...
7.2K
Hormonal Regulation of the Menstrual Cycle
297
The ovarian cycle regulates endometrial changes throughout a single menstrual cycle via the coordinated action of gonadotrophin-releasing hormone (GnRH) and gonadotrophins.
At puberty, GnRH begins a pulsatile release pattern, which triggers the anterior pituitary gland to secrete follicle-stimulating hormone (FSH) and luteinizing hormone (LH). The frequency and amplitude of GnRH pulses vary across the menstrual cycle, with faster pulses favoring LH release and slower pulses favoring FSH...
At puberty, GnRH begins a pulsatile release pattern, which triggers the anterior pituitary gland to secrete follicle-stimulating hormone (FSH) and luteinizing hormone (LH). The frequency and amplitude of GnRH pulses vary across the menstrual cycle, with faster pulses favoring LH release and slower pulses favoring FSH...
297

