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Updated: Jun 7, 2025

"Phagosome Closure Assay" to Visualize Phagosome Formation in Three Dimensions Using Total Internal Reflection Fluorescent Microscopy TIRFM
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μMap-Interface:时间光近距离标记识别F11R作为暂时细胞表面组的功能成员

Sean W Huth1,2, Jacob B Geri1,2, James V Oakley1,2

  • 1Merck Center for Catalysis at Princeton University, Princeton, New Jersey 08544, United States.

Journal of the American Chemical Society
|November 12, 2024
PubMed
概括

我们开发了一种新的方法,即μMap-Interface, 用于绘制参与细胞形成的蛋白质,

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Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
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