在 Bacillus licheniformis 中以转录学为指导的理性工程来增强使用未经处理的糖蜜的聚-γ-胺酸生物合成
Rui Han1, Qian Zhong1, Yifan Yan1
1College of Food Science and Light Industry, Nanjing Tech University, Nanjing 211816, China; State Key Laboratory of Materials-oriented Chemical Engineering, Nanjing Tech University, Nanjing 211816, China.
International journal of biological macromolecules
|November 12, 2024
概括
这项研究设计了Bacillus licheniformis以有效地从未经过处理的糖蜜中产生聚-γ-胺酸 (γ-PGA). 代谢修改显著提高了γ-PGA产量,证明了工业应用的成本效益高的生物转化.
科学领域:
- 生物技术是生物技术.
- 微生物合成 微生物合成
- 代谢工程是代谢工程.
背景情况:
- 聚-γ-胺酸 (γ-PGA) 是一种具有多种应用的可生物降解聚合物.
- 在 γ-PGA 生产中使用诸如糖蜜之类的非食品原料提供了一个可持续的替代方案.
- 糖蜜的复杂成分可能会阻碍微生物转化效率.
研究的目的:
- 增强Bacillus licheniformis将未经处理的糖蜜转化为γ-PGA的能力.
- 使用转录组分析指导代谢工程策略.
- 为了实现具有成本效益和高产量的γ-PGA生物合成.
主要方法:
- 在糖蜜上种植的Bacillus licheniformis的转录基因分析.
- 代谢工程策略包括基因淘汰 (AlsS,CcpA) 和基因过度表达.
- 使用未经处理的糖蜜作为唯一的碳来源优化发酵.
主要成果:
- 转录组数据显示基质利用,副产品合成和前体合成模块中的基因表达改变.
- 淘汰AlSS和CcpA减少了副产品的形成,并减轻了碳催化剂的抑制.
- 前体基因的双重过度表达,结合淘汰,导致γ-PGA标位为48.26g/L.
- 与野生类型菌株相比, γ-PGA 产量增加了 3.12 倍.
结论:
- 由转录学指导的代谢工程可以显著改善未经处理的糖蜜的γ-PGA生产.
- 开发的菌株和工艺为工业 γ-PGA 合成提供了一条具有成本效益的途径.
- 这项研究为优化复杂的非食品原料的微生物生物转化提供了宝贵的见解.
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