三重获取质谱法 (TRAM) 在一次运行中结合了向和非向的代谢学
Lisa Panzenboeck1, Harald Schoeny2, Bruno Stelzer2
1Department of Analytical Chemistry, Faculty of Chemistry, University of Vienna, Waehringer Str. 38, 1090, Vienna, Austria; Vienna Doctoral School in Chemistry (DoSChem), University of Vienna, Waehringer Str. 42, 1090, Vienna, Austria.
Analytica chimica acta
|November 12, 2024
概括
我们开发了TRAM,这是一种用于质谱的三重获取策略,融合了非向和向的代谢学. 这种方法实现了高通量,准确的量化,同时降低了样本需求和成本.
科学领域:
- 分析化学 分析化学
- 代谢学 代谢学 代谢学
- 质谱测量质量谱测量
背景情况:
- 推出TRAM,这是一个三重采购战略,用于高速四极飞行时间质谱仪.
- 在一个单一的分析运行中,TRAM将非目标和目标代谢组合在一起.
- 结合了全扫描MS1,数据依赖MS2 (DDA) 和有针对性的计划MS2 (MRM) 在~1s周期内获取.
研究的目的:
- 将非目标和目标代谢学分析整合到一个单一的,高效的工作流中.
- 为了利用高分辨率质谱仪进行全面的代谢分析.
- 为了验证TRAM战略对定量代谢学的表现.
主要方法:
- 采用水友互动液体染色学 (HILIC) 工作流程.
- 生成的染色体保留时间和针对性MRM实验的优化条件.
- 在~1秒的测量周期内获得全扫描MS1,顶部30DDAMS2和计划MRMMS2数据.
主要成果:
- 通过TRAM,可以证明线性工作范围和量化极限与仅使用MRM的方法相比.
- 在SRM 1950人类血参考材料中实现了准确的量化.
- 对于非向代谢物质,与仅用于DDA的方法表现出相当性,在人类脑脊液中应用.
结论:
- 通过TRAM,可以进行深入的代谢分析,覆盖范围和吞吐量都很高.
- 提供黄金标准的绝对量化到低纳米分子度.
- 减少所需的样本量,分析时间,成本和环境影响.
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