在六倍形小麦中对抗细菌叶条的QTLs的遗传映射
Krishna Acharya1, Zhaohui Liu2, Jeffrey Schachterle3
1Department of Plant Sciences, North Dakota State University, Fargo, ND, 58105, USA.
概括
研究人员在小麦中确定了强大的定量特征位点 (QTLs),用于抗细菌叶条 (BLS) 耐药性. 这些QTL位于染色体3B,5A和7D上,可用于开发抗BLS的小麦品种.
科学领域:
- 植物遗传学 植物遗传学
- 农作物科学 农作物科学
- 植物病理学 植物病理学
背景情况:
- 细菌叶条纹 (BLS),是由Xanthomonas透光的pv引起的. 无粉,对全球小麦产量构成重大威胁.
- 在种植的六倍形小麦品种中,高水平的抗BLS是不常见的.
- 识别抗药性的新型遗传来源对于开发耐药小麦至关重要.
研究的目的:
- 选多种小麦基因型,以寻找对细菌叶状菌株耐药性的来源.
- 通过使用重组杂交种群来研究BLS耐药性的遗传基础.
- 识别和绘制数量特征位点 (QTLs),赋予BLS耐药性.
主要方法:
- 在温室条件下选101种小麦基因型以检测BLS耐药性.
- 在温室和实地试验中开发和评估两种复合同血统种群 (BoostND和ITMI).
- 定量特征位置 (QTL) 映射和分析表型变异.
- 将已识别的QTL相关标记物转换为用于标记物辅助选择的KASP标记物.
主要成果:
- 五种小麦品种,包括品种Boost和合成品种W-7984,表现出良好的BLS耐受性.
- 在BoostND群体的染色体3B和5A上,以及在ITMI群体的染色体7D上绘制了BLS抗性的强大的QTL.
- 3B QTL解释了多达38%的表型变异 (温室),而5A QTL解释了多达29% (田野),7D QTL解释了多达46% (温室).
- 观察到BLS严重程度和头条数天之间存在负相关性,这表明成熟度和阻力之间存在联系.
结论:
- 强大的QTLs赋予细菌叶子串状抗性被成功地映射到小麦染色体3B,5A和7D.
- 这些已识别的QTL代表了培育抗BLS小麦品种的宝贵遗传资源.
- 开发KASP标记器将促进这些抗性QTLs进入精英小麦生产线.
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