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Electron Microscope Tomography and Single-particle Reconstruction

Electron Microscope Tomography and Single-particle Reconstruction

Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...

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高分辨率的细节纤维素结构通过电印.

Farnaz Rezaei¹, Daniel O Carlsson², Jimmy Hedin Dahlstrom²

¹Department of Materials Science and Engineering, Uppsala University, 75105, Uppsala, Sweden. Farnaz.rezaei@angstrom.uu.se.

Scientific reports

|November 12, 2024

概括

电子打印精确地将聚合物纤维定位为3D结构. 这种技术制造出具有亚微米特性的定制分离膜,尽管打印速度需要改进.

关键词:

增材制造增材制造是一种增材制造.纤维素乙酸盐的使用情况电子打印是一种电子打印技术.高分辨率的3D打印技术

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相关实验视频

Last Updated: May 13, 2026

Creating Transient Cell Membrane Pores Using a Standard Inkjet Printer

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Published on: March 16, 2012

3D Printed Porous Cellulose Nanocomposite Hydrogel Scaffolds

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Biomimetic Replication of Root Surface Microstructure using Alteration of Soft Lithography

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科学领域:

材料科学材料科学材料科学
化学工程是化学工程的重要组成部分.
生物材料工程生物材料工程

背景情况:

电制造微/纳米级聚合物纤维,但缺乏精确的纤维定位.
电中的有限定位精度阻碍了复杂的设计结构的创建.

研究的目的:

开发一种电印技术,以加强对纤维放置的控制.
用电印研究微米精度的3D结构的制造.
探索电印技术在制造定制分离膜方面的潜力.

主要方法:

通过减少喷嘴-采集器距离来改进纤维定位控制,开发了一种电印技术.
使用纤维素酸盐 (CA) 作为3D膜打印的生物材料.
评估参数包括CA度,分子量,打印速度,图案和应用电压.

主要成果:

实现了对纤维定位的改进控制,使得微米级设计的3D结构的制造成为可能.
证明了印刷结构的可能性,纤维间距离只有3微米.
通过优化电印参数在亚微米尺度上获得纤维直径.

结论:

电子打印为制造定制的分离膜提供了一个有前途的方法,具有高精度.
优化的打印参数允许对纤维直径和间距进行显著的控制.
印刷速度仍然是进一步推进电印技术的关键挑战.