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在vivo中定量可视化H₂S₂的NIR-II光/光声学双相径测探头具有独特的识别位点

Bangping Hu¹, Qinian Liu¹, Yulan Jiang¹

¹State Key Laboratory for Chemistry and Molecular Engineering of Medicinal Resources, Key Laboratory for Chemistry and Molecular Engineering of Medicinal Resources (Ministry of Education of China), Collaborative Innovation Center for Guangxi Ethnic Medicine, School of Chemistry and Pharmaceutical Sciences, Guangxi Normal University, Guilin, 541004, P. R. China.

Angewandte Chemie (International ed. in English)

|November 12, 2024

概括

研究人员开发了一种新型探针,用于对硫化 (H2S2) 的深层组织成像. 这种工具可以在体内精确,定量地可视化H2S2,进步氧化还原生物学和疾病研究.

科学领域:

生物医学成像技术生物医学成像技术
化学生物学化学生物学
转毒生物学转毒生物学

背景情况:

硫化 (H2S2) 在氧化还原生物学和信号传导中至关重要.
在深层组织中精确的体内可视化H2S2对于了解病理生理过程至关重要.
现有的H2S2探头缺乏特异性和深层组织透能力.

研究的目的:

开发一种用于深层组织中 H2S2 的定量可视化新型探针.
为了克服当前探测器在选择性和成像深度方面的局限性.
为了建立一个新的H2S2介导反应,用于探头开发.

主要方法:

开发了一个近红外II (NIR-II) 光和光声学 (PA) 双比度探针 (NIR-II-H2S2).
用一种独特的H2S2介导的二硫醇形成反应来构造探头.
在实验室和体内使用肝损伤小鼠模型验证了探针的性能.

主要成果:

对于H2S2.2,NIR-II-H2S2探头表现出高的特异性和灵敏性 (1.8 nM) 对于H2S2.
在NIR-II光 (107倍) 和PA (6.5倍) 两种情况下都取得了显著的双比度反应.
在老鼠模型的深层组织中,成功地展示了波动的H2S2水平的定量成像.

结论:

开发的NIR-II-H2S2探头是体内H2S2可视化的一个有前途的工具.
这种探测器可以在病理生理条件下准确量化H2S2.
新的识别站点为设计未来的H2S2成像剂提供了潜力.

关键词:

双重比率计的比率计.通过H2S2介导的滴醇形成.多硫化是多硫化的一种.在NIR-II中,我们可以看到NIR-II.定量检测检测量检测量检测量检测量检测量检测量

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