科尔迪塞可以通过腺素受体改善过度激活和体反应,在试验室内人类精子电容化期间可以通过腺素受体进行
Lijun Shan1,2, Linmei Gao1,2, Yuhao Chai1,2
1School of Pharmacy, Hangzhou Medical College, Hangzhou, Zhejiang, China.
Reproductive biology and endocrinology : RB&E
|November 13, 2024
概括
科尔迪塞宾通过通过腺素受体通路提高人体精子的运动性和体反应来增强精子容量. 这种化合物显示出辅助生殖技术和动物育种的潜力.
科学领域:
- 生殖生物学 生殖生物学
- 分子药理学分子药理学
- 精子生理学 精子生理学
背景情况:
- 精子电容对于受精是必不可少的,使精子过度激活和卵细胞透的青体反应成为可能.
- Kordycepin 是 Cordyceps militaris 中的一种腺类型,具有各种生理活动,但其在精子电容中的作用尚不清楚.
- 这项研究调查了cordycepin对人类精子容量的影响和潜在机制.
研究的目的:
- 为了确定cordycepin对人类精子容量的影响.
- 阐明康迪塞影响精子电容性的分子机制,重点关注腺受体.
主要方法:
- 在 in vitro 电容化过程中,人类精子被用不同度的 cordycepin 治疗.
- 精子的运动性,过度活化和体反应使用专门的测试来评估.
- 分析了蛋白激酶A (PKA) 活性,氨酸酸化,DNA完整性和活性氧物种 (ROS);使用抑制剂和分子对接证实了腺素受体表达和功能.
主要成果:
- 科迪塞平显著增强过度激活的精子运动性和体反应,同时增加了PKA活性和蛋白质氨酸酸化.
- 科迪塞宾并没有影响精子ROS水平或DNA损伤.
- 在人类精子中发现了四种腺受体亚型;它们的抑制减少了cordycepin的积极作用,分子对接证实了cordycepin与这些受体的结合.
结论:
- 科尔迪塞通过腺受体介导的信号通路促进人类精子电容.
- 这些发现表明cordycepin在辅助生殖技术和动物育种应用中的潜在实用性.
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