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分子检测方法的比较,用于诊断非结核性真菌菌感染

Leilei Wang¹, Yu Chen¹, Qingqing Wang¹

¹Department of Infectious Diseases, Zhongshan Hospital of Fudan University, Shanghai, 200032, China.

European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology

|November 13, 2024

概括

准确识别非结核性真菌菌 (NTM) 是至关重要的. 大基因组下一代测序 (mNGS),向下一代测序 (tNGS) 和多重PCR显示出快速NTM疾病诊断的希望.

科学领域:

医学诊断医学诊断医学诊断
微生物学微生物学
基因组学就是基因组学.

背景情况:

准确识别非结核菌 (NTM) 对于有效的患者管理至关重要,但仍然具有挑战性.
包括高通量测序在内的新型分子技术正在开发中,以改善NTM检测.

研究的目的:

评估基于放大功率的向高通量测序和对NTM感染的元基因学无偏高通量测序的诊断价值.
为了比较多重聚合酶连锁反应 (多重PCR),元基因组下一代测序 (mNGS),向下一代测序 (tNGS) 和向纳米孔子测序 (tNanopore) 在识别NTM物种的性能.

主要方法:

分析了115个来自NTM感染确诊患者的临床样本.
多PCR,mNGS,tNGS和tNanopore用于病原体检测的应用.
在分子技术中比较阳性率和物种识别准确度.

主要成果:

对于NTM感染诊断的敏感度为mNGS的44.3%,tNGS的42.6%,多PCR的36.5%. 综合灵敏度达到了54.8%.
在mNGS,tNGS和多PCR中,物种级别的鉴定一致性为80.6%,在亚种级别的匹配率为45.2%.
在tNanopore,tNGS和mNGS之间的物种级别识别协议为73.3%.

结论:

分子测定,包括mNGS和tNGS,在精确的NTM物种识别中表现相似.
这些技术显示出作为NTM疾病快速诊断的高效和替代工具的巨大潜力.
进一步的验证可能会将这些方法确立为NTM感染的标准诊断方法.

关键词:

标识识别识别识别下一代元基因组测序的测序.多重PCR是一种多重PCR 非结核性菌根杆菌是没有结核的.有针对性的纳米孔测序.有针对性的下一代测序.

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