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在单细胞中的个体基因内源性RNA G-四重复的可视化

Yicong Dai1,2,3,4, Xucong Teng1,2,3,4, Hongwei Hou1,2,3,4

  • 1Beijing Life Science Academy, Beijing, China.

Methods in molecular biology (Clifton, N.J.)
|November 13, 2024
PubMed
概括

本研究介绍了模块组装多功能探针试验 (MAMPA) 用于在单细胞中可视化RNA G-四重复合体 (G4s). 这种新方法可以准确地检测单个基因内的特定RNA G4结构,从而推进RNA生物学研究.

关键词:
光探测器探测器可以发光.在G-quadruplex中使用.单一的G-四重复检测检测的G-四重复检测核酸模板化生物正对角反应RNA的二级结构是RNA的二级结构.滚动圈放大器 滚动圈放大器一个单细胞成像技术.

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Ribosome Profiling02:24

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique helps...