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Updated: Jun 7, 2025

PAR-CliP - A Method to Identify Transcriptome-wide the Binding Sites of RNA Binding Proteins
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PAR-CliP - A Method to Identify Transcriptome-wide the Binding Sites of RNA Binding Proteins

Published on: July 2, 2010

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用5'单酸盐依赖的外核酶消化和RNA测序来对RNA的5'盖状况进行全转录组分析

Maxime Wery1, Ugo Szachnowski2, Sara Andjus2

  • 1ncRNA, Epigenetic and Genome Fluidity, CNRS UMR3244, Sorbonne Université, PSL University, Institut Curie, Paris, France. maxime.wery@curie.fr.

Methods in molecular biology (Clifton, N.J.)
|November 13, 2024
PubMed
概括

这项研究引入了一种方法来评估RNA盖状况的全转录组范围. 它使用外核酶来降解切割的RNA,通过RNA测序来分析完整的N7-甲基瓜诺辛 (m7G) 封闭的RNA.

关键词:
在Dcp2中,我们可以看到Dcp2.脱皮是为了脱皮.在RNA-Seqq.核糖体RNA的枯竭是指核糖体RNA的枯竭.终结者 5′-酸盐依赖的外核酶Xrn1敏感的不稳定转录在mRNA衰变过程中.

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