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Updated: Jun 7, 2025

Studying Ribonucleotide Incorporation: Strand-specific Detection of Ribonucleotides in the Yeast Genome and Measuring Ribonucleotide-induced Mutagenesis
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Studying Ribonucleotide Incorporation: Strand-specific Detection of Ribonucleotides in the Yeast Genome and Measuring Ribonucleotide-induced Mutagenesis

Published on: July 26, 2018

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RNA衰变试验:5-乙-氨酸标记和追逐

Haripriya Gupta1, Jayden R Lee1, Kane B Hoffman1

  • 1Department of Oncology Science, University of Oklahoma, Oklahoma City, OK, USA.

Methods in molecular biology (Clifton, N.J.)
|November 13, 2024
PubMed
概括

这项研究引入了5-乙烯尿氨酸标签,以测量RNA衰变动力学. 这种方法提供了RNA稳定性和真核生物中的基因表达调节的动态视图.

关键词:
代谢标签的标记在mRNA衰变过程中.qPCRR 是一个很好的方法.

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A Reporter Based Cellular Assay for Monitoring Splicing Efficiency

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相关概念视频

Maxam-Gilbert Sequencing01:05

Maxam-Gilbert Sequencing

In the same year as the discovery of the Sanger sequencing method, another group of scientists, Allan Maxam and Walter Gilbert, demonstrated their chemical-cleavage method for DNA sequencing. The Maxam-Gilbert method relies on using different chemicals that can cleave the DNA sequence at specific sites, the separation of resulting DNA fragments of variable size using electrophoresis, and deciphering the DNA sequence from the resulting gel bands.
Challenges of the Maxam-Gilbert Method
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