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Updated: Jul 18, 2026

Novel RNA-Binding Proteins Isolation by the RaPID Methodology
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reCRAC:一种严格的方法,用于精确地绘制酵母中的蛋白质-RNA相互作用

Michaela Ristová1, Vadim Shchepachev2, David Tollervey3

  • 1Wellcome Centre for Cell Biology, University of Edinburgh, Edinburgh, UK. s1507601@ed.ac.uk.

Methods in molecular biology (Clifton, N.J.)
|November 13, 2024
PubMed
概括

我们开发了reCRAC,这是一种研究RNA结合蛋白 (RBPs) 和RNA相互作用的新方法,即使是与不稳定的蛋白质. 这种技术提高了准确性,并减少了蛋白质降解,以获得更好的研究见解.

关键词:
CRAC (cDNA的交叉链接和分析)变质拉下拉下和净化净化蛋白RNA相互作用减少了蛋白质降解的发生.紫外线交叉连接的UV交叉连接这就是reCRAC (反向CRAC).RNA结合蛋白质是RNA结合的蛋白质.

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相关概念视频

Ribosome Profiling02:24

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique helps...