相关实验视频

Updated: Jun 7, 2025

Selection-dependent and Independent Generation of CRISPR/Cas9-mediated Gene Knockouts in Mammalian Cells
11:35

Selection-dependent and Independent Generation of CRISPR/Cas9-mediated Gene Knockouts in Mammalian Cells

Published on: June 16, 2017

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选择修复淘汰:通过可选择的同质导向DNA修复,有效的无PTC基因淘汰

Michael A Cortázar1, Sujatha Jagannathan2,3

  • 1Department of Biochemistry and Molecular Genetics, University of Colorado Anschutz Medical Campus, Aurora, CO, USA.

Methods in molecular biology (Clifton, N.J.)
|November 13, 2024
PubMed
概括

这项研究引入了一种新的CRISPR/Cas9基因淘汰方法,可以避免过早终止子 (PTC). 这种SelectRepair Knockout策略简化了查和验证,提供了一种更可靠的方法来实现基因功能丧失.

关键词:
基因编辑CRISPR/Cas9基因编辑同质导向维修是同质导向维修的一种方式.这是一次Knockout.这是一个NMD.无稽之谈中介的mRNA衰变是没有意义的.

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