在13个欧洲国家的人类,动物和食品中Clostridioides difficile核型和序列类型的分布
Maja Rupnik1,2,3, Virginie Viprey4, Sandra Janezic1,2
1National Laboratory for Health, Environment and Food, Maribor, Slovenia.
Emerging microbes & infections
|November 13, 2024
概括
困难菌,一个健康的病原体,在欧洲人类,动物和食物来源中显示出不同的核型和序列类型. 一些核糖型表明与食物或动物传播的联系,在人类中发现的罕见,与食物相关的新兴菌株.
科学领域:
- 微生物学与传染病的研究
- 基因组学和分子流行病学
- 一个健康监测
背景情况:
- 困难菌是一种重要的One Health病原体,影响人类,动物和环境的健康.
- 现有的One Health对C. difficile的研究往往在地理上或水库上受到限制.
- 食品被认为是C. difficile的潜在传播途径.
研究的目的:
- 综合分析来自欧洲的人类,动物和食品来源的C. difficile分离物.
- 使用全基因组测序 (WGS) 描述C. difficile核型 (RT) 和序列类型 (ST) 的多样性.
- 调查来自不同来源的C. difficile菌株之间的潜在传播途径和遗传关系.
主要方法:
- 在13个欧洲国家收集了441个来自人类 (n=280),动物 (n=96) 和食品 (n=65) 的C. difficile分离物.
- 使用核型,毒素型和全基因组测序 (WGS) 的分析.
- 序列类型 (ST),核型 (RT) 和密码类的识别和表征.
主要成果:
- 确定了83种序列类型 (ST),其中ST11 (80个分离物) 和ST1 (54个分离物) 是最常见的.
- 检测到106个核型 (RTs),其中10个共享到所有三个来源,68个来源特定.
- 发现了特定RTs与食品或动物传播途径之间的潜在关联;发现了来自新兴CI-III类的罕见,与食品相关的C. difficile菌株.
结论:
- 在欧洲的人类,动物和食物储备中,C. difficile在线核型和序列类型中表现出显著的多样性.
- 某些核糖类型可能表明特定的传播途径,特别是涉及食品和动物.
- 人类中新出现的C. difficile菌群很少见,似乎与食物相关.
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