探索来自欧洲选定的国家入侵性血清组B脑膜炎球菌菌株中的H因子结合蛋白的序列多样性和表面表达
Stephen A Clark1, Laura Willerton1, Heike Claus2
1Meningococcal Reference Unit, UK Health Security Agency, Manchester Royal Infirmary, Manchester, UK.
Human vaccines & immunotherapeutics
|November 13, 2024
概括
大多数侵入性B组脑膜炎球菌菌株表达高水平的H结合因子蛋白 (fHbp),对疫苗有效性至关重要. 对451个菌株的分析显示,92%的疫苗超过了MenB-fHbp疫苗疗效的值.
科学领域:
- 微生物学 微生物学
- 疫苗学 疫苗学 疫苗学
- 免疫学 免疫学 免疫学
背景情况:
- H因子结合蛋白 (fHbp) 对于像MenB-fHbp这样的脑膜炎球菌疫苗至关重要.
- 高细菌表面fHbp表达对于疫苗诱导的抗体有效性至关重要.
- 测量试验量化fHbp表达,平均光强度 (MFI) >1000预测细菌死亡.
研究的目的:
- 评估fHbp的分布和表达水平在侵袭性B组脑膜炎球菌菌株.
- 评估不同欧洲地区的MenB-fHbp疫苗预测覆盖率.
主要方法:
- 分析了2016年从英格兰,威尔士,北爱尔兰,德国,意大利和西班牙收集的451种侵入性B组脑膜炎球菌菌株.
- 使用流量细胞计测量测量测试测量fHbp表达的量化.
- 对fHbp分布和克隆复合物的评估.
主要成果:
- 在所有分析的菌株中,92%的fHbp表达在MFI 1000值以上,这表明可能对MenB-fHbp疫苗诱导的抗体敏感.
- 菌株分布和fHbp表达在英格兰,威尔士,北爱尔兰,德国和意大利 (92.1%-94.6%覆盖率) 都相似.
- 西班牙的预测覆盖率较低 (84%),原因是表达率较低的菌株的比例较高,特别是来自克隆复合体213.
结论:
- 大多数侵袭性B组脑膜炎球菌菌株表达足够的fHbp,以有效地覆盖MenB-fHbp疫苗.
- 包括fHbp基因的上游基因间序列改善了疫苗覆盖率的基因型预测.
- 需要进一步的MEASURE分析来完善对不太常见的fHbp变体的预测.
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