梅萨拉胺载 ethyl纤维素纳米颗粒:优化和体内评估抗氧化剂潜力在性结肠炎
Preety Gautam1,2, Md Habban Akhter1, Anubhav Anand2
1Faculty of Pharmacy, DIT University, Mussoorie, Diversion Road, Makkawala, Uttarakhand 248009, India.
Biomedical materials (Bristol, England)
|November 13, 2024
概括
优化的梅沙胺纳米颗粒显示出对性结肠炎治疗有前途的抗氧化作用. 该研究详细介绍了配方,体内抗氧化剂的潜力,以及有效的结肠药物向的稳定性.
科学领域:
- 制药科学 制药科学
- 纳米技术 纳米技术
- 药物运输 药物运输 药物运输
背景情况:
- 性结肠炎 (UC) 需要有针对性的药物治疗才能有效治疗.
- 梅萨拉胺 (MES) 是UC的关键治疗剂,但其有效性可能受到交付挑战的限制.
- 纳米粒子配方为改善MES交付和治疗结果提供了潜力.
研究的目的:
- 使用Box-Behnken设计优化带有梅沙胺 (MES) 的乙烯纤维素纳米颗粒 (EC-NPs),用于结肠特异性药物输送.
- 在相关模型中研究优化MES-NP的体内抗氧化潜力.
- 评估开发的MES-NPs的稳定性和释放特性.
主要方法:
- 使用油/水乳液溶剂蒸发技术制备MES-NP.
- 通过Box-Behnken设计优化配方参数 (聚合物度,表面活性剂度,超声波持续时间).
- 对NP的表征 (粒子大小,泽塔潜力,多分散性指数,通过SEM的形态).
- 在模拟的胃和肠液中进行体外药物释放研究.
- 在体内评估抗氧化剂潜力 (谷氨,SOD,LPO水平).
- 在不同的温度和湿度条件下进行长期稳定性研究.
主要成果:
- 优化的MES-NPs的粒子大小为142 ± 2.8 nm,泽塔电位为-24.8 ± 2.3 mV,捕获效率为87.9 ± 1.6%,PDI为0.226 ± 0.15.
- 扫描电子显微镜证实了NP的均和球形形态.
- 实验室释放显示,在模拟的胃介质中发生突发释放,随后在肠液中持续释放,最符合科尔斯迈耶-佩帕斯模型 (R2=0.962).
- 在各种储存条件下,该配方在六个月内表现出良好的稳定性.
- 在体内研究表明,与对照组相比,谷氨和SOD水平显著增加,LPO水平降低.
结论:
- 优化的乙纤维素纳米颗粒有效地封装梅萨拉,用于向结肠输送.
- 开发的MES-NP具有显著的体内抗氧化潜力,有利于性结肠炎的管理.
- 该配方表现出有利的物理化学特性,释放动力学和稳定性,表明它对性结肠炎的治疗有希望.
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