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抗原胺类蛋白质及其DNA结合蛋白质中微塑料诱导的分子变化的毒理效应和潜在的生殖风险

Carmela Marinaro¹, Giulia Scarciello¹, Anna Rita Bianchi¹

¹Department of Biology, University of Naples Federico II, Via Cinthia, 21, 80126, Naples, Italy.

Chemico-biological interactions

|November 13, 2024

概括

在 (Mytilus galloprovincialis) 中,聚乙烯微塑料 (PS-MPs) 暴露改变了精子染色蛋白的蛋白质,可能损害生殖健康. 较高的PS-MP度增加了DNA保护,但阻碍了受精所需的蛋白质释放.

科学领域:

海洋生物学海洋生物学
环境毒理学环境毒理学
生殖毒理学生殖毒理学

背景情况:

塑料污染,特别是聚烯微塑料 (PS-MPs),对海洋生态系统构成重大威胁.
像Mytilus galloprovincialis这样的类是海洋环境健康的关键指标.
了解PS-MPs对海洋无脊椎动物的生殖毒理影响至关重要.

研究的目的:

研究PS-MPs对Mytilus galloprovincialis对生殖健康的影响.
分析PS-MPs对精子染色质原胺类 (PL) 蛋白质的影响.
评估游戏性可塑性和生殖损害的潜在分子机制.

主要方法:

将Mytilus galloprovincialis暴露在两个度的PS-MPs (0.5和1μg/L) 中.
使用SDS-PAGE.分析原胺类 (PL) 蛋白质电泳模式.
测量PL的DNA结合和对氧化损伤的保护.
对PL聚-ADP-ribosylation的研究.

主要成果:

暴露于PS-MP改变了PL蛋白模式,并增加了它们结合和保护DNA的能力,特别是在1μg/L时.
在更高的PS-MP度下,从精子核中减少了PLs的释放.
在暴露于PS-MP后,观察到PL-II的增多多ADP核糖化,这可能会影响DNA结合.

结论:

暴露在1μg/LPS-MPs中的鱼在精子染色蛋白中表现出分子变化,这表明了性质可塑性.
这种程度的PS-MP暴露可能会损害Mytilus galloprovincialis的生殖健康,因为它会阻碍受精期间的必需蛋白质释放.

关键词:

微塑料是一种微塑料.污染污染污染复合 (ADP) - 基化聚乙烯是一种聚乙烯.类似于蛋白质胺的蛋白质胺类药物精子染色素的精子染色素是什么

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