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来自Nostoc公社的免疫调节多糖的结构阐明和分子机制

Yiting Yang¹, Ping Ren¹, Ying Sun¹

¹College of Life Sciences, Engineering Research Center of Bioreactor and Pharmaceutical Development, Ministry of Education, Jilin Agricultural University, Changchun 130118, Jilin, China.

International journal of biological macromolecules

|November 13, 2024

概括

诺斯托克公社的多糖体NCVP2通过调节氧化和活性氧物种来增强免疫力. 它针对多个免疫路径,显示出健康食品开发的潜力.

科学领域:

物理学的生理学
免疫学免疫学免疫学
生物化学生物化学

背景情况:

诺斯托克公社 (蓝绿藻) 多糖 (NCVP) 具有已知的生物活动.
关于NCVP的具体机制和结构-活动关系仍然未被充分探索.
在全球范围内,NCVP在食品和传统医学中得到了广泛的应用.

研究的目的:

为了阐明一个来自Nostoc社的新型多糖体的结构和免疫调节机制.
为了研究这种多糖的发展到健康食品的潜力.

主要方法:

使用 DEAE-52 和 Sephadex G-100 染色学进行序列净化.
通过甲基化和核磁共振 (NMR) 进行结构分析.
在体外免疫调节试验 (氧化,反应性氧物种),RNA测序 (RNA-seq),基因本体学 (GO) 和基因和基因组的京都百科全书 (KEGG) 途径分析,以及西式涂抹.

主要成果:

一种新型的多糖,NCVP2 (135 kDa),由α-D-Galp,β-D-Glcp和α-D-Xylp残留物组成,被分离出来.
NCVP2通过增加氧化 (NO) 和活性氧物种 (ROS) 生产,显示出强大的体外免疫调节活性.
RNA-seq确定了2048个差异表达基因 (DEGs),涉及免疫信号通路,包括MAPK和Toll-like受体 (TLR).
西方斑点证实NCVP2与TLR2和TLR4/MD2相互作用,调节TLR7/IRF7,MAPK和PI3K/AKT通路.

结论:

通过多种途径和点,NCVP2表现出显著的免疫调节效应.
这种新型的多糖具有在功能性食品和免疫调节剂中应用的巨大潜力.

关键词:

这是一种免疫调节剂.诺斯托克 (Nostoc) 公社沃赫 (Vaucher) 是一个公社.它是一种多糖化合物.收费类受体的收费类受体文字转录学 (Transcriptomics) 是一个学科.

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