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染色体尺度上的基因组组装,用于珍珠斑,Etroplus suratensis的端粒末

Vinaya Kumar Katneni¹, Karthic Krishnan², Sudheesh K Prabhudas²

¹Centre for Bioinformatics, Nutrition Genetics and Biotechnology Division, ICAR - Central Institute of Brackishwater Aquaculture, No 75, Santhome High Road, MRC Nagar, Chennai, 600 028, Tamil Nadu, India. Vinaya.Katneni@icar.gov.in.

Scientific data

|November 13, 2024

概括

对珍珠斑鱼的基因组进行了测序,揭示了它对不同盐度的显著适应性. 这种遗传资源有助于理解鱼的气候弹性.

科学领域:

基因组学就是基因组学.
水生生物学水生生物学
进化生物学进化生物学

背景情况:

珍珠斑 (Etroplus suratensis) 是一种以其异常适应各种盐度水平而闻名的鱼,从淡水到海洋环境.
了解这种盐分耐受性的遗传基础对于预测鱼类适应不断变化的气候条件至关重要.

研究的目的:

为了生成第一个高质量的珍珠斑鱼 (Etroplus suratensis) 的基因组组合.
为研究这种物种盐度适应的机制提供基因组资源.

主要方法:

使用PacBio HiFi和Arima HiC测序技术生成了高质量的初级和分阶段基因组组件.
基因组组装质量被评估为连续性,完整性 (BUSCO) 和染色体定.
预测了编码蛋白质的基因,并对其功能进行了注释.

主要成果:

产生了一个高度连续的初级基因组组件 (连续N50: 36 Mb) 和一个分阶段组件 (1.247 Gb,N50: 51.57 Mb).
最终的组装将98%的基因组固定在24个染色体上,并且完成了99.9%.
预测了27,192个编码蛋白质的基因,其中21,580个功能性注释.

结论:

产生的珍珠点基因组组合是未来研究的高质量资源.
这些基因组数据将有助于研究鱼适应盐度和适应气候变化的基因基础.

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