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热性失活的肺炎性菌增加了斑马鱼细胞中昼夜钟基因表达

Camila Morales Fénero¹, Raina E Sacksteder¹, Andrew G Diamos¹

¹Department of Microbiology and Environmental Toxicology, University of California Santa Cruz, Santa Cruz, USA.

Scientific reports

|November 13, 2024

概括

细菌暴露会改变斑马鱼的时钟基因表达,受到光线的影响. 热杀死性肺炎菌 (HK-Spn) 调高核心抑制因子,其影响因光照条件和活性氧物种而有所不同.

科学领域:

时间生物学时间生物学
分子生物学分子生物学
免疫学免疫学免疫学

背景情况:

昼夜时钟调节日常节律,受到环境线索的影响,如光.
感染可以破坏昼夜基因表达,光调节这些效应在teleosts.
细菌暴露对斑马鱼钟基因调节的直接影响仍然没有被描述.

研究的目的:

为了研究细菌暴露对斑马鱼昼夜钟基因表达的直接影响.
为了确定光线条件对细菌诱导的时钟基因改变的影响.

主要方法:

使用斑马鱼Z3细胞进行实验分析.
在不同的光照条件下 (持续的黑暗和光照) 暴露于热杀死性肺炎菌 (HK-Spn) 的细胞.
评估了核心昼夜钟基因 (cry1a,per3,per1b,per2) 的表达.
使用H2O2和N-乙半氨酸研究了活性氧物种 (ROS) 的作用.

主要成果:

在恒定的黑暗中,HK-Spn暴露上调了核心抑制因子 (cry1a,per3,per1b).
在光线下,HK-Spn快速上调 per2 和 cry1a,具有强度依赖的效应.
ROS清除剂N-乙囊抑制了HK-Spn诱导的per2,cry1a和per3.3的增大.

结论:

不活跃的细菌暴露显著影响斑马鱼钟基因表达.
光强度和细菌存在的相互作用调节昼夜基因调节.
活性氧物种通过细菌暴露调解了时钟基因表达的增强.

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