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检测和分子特征的耐卡巴胺抗性格拉姆阴性细菌分离物

Aliaa A Mustafa¹, Hala Abushady¹, Reda Roshdy²

¹Department of Microbiology, Faculty of Science, Ain Shams University, Cairo, Egypt.

|November 13, 2024

概括

这项研究评估了对抗卡巴尼姆抗性格拉姆阴性细菌 (CRGNB) 的诊断工具. HiCrome KPC agar对CRGNB检测具有很高的敏感性,有助于在医院快速诊断和感染控制.

科学领域:

微生物学与传染病的研究
临床诊断临床诊断临床诊断
细菌基因组学细菌基因组学

背景情况:

抗微生物药物耐药细菌 (ARB),特别是耐卡巴胺的抗грам阴性细菌 (CRGNB),对全球健康构成重大威胁,增加死亡率和发病率.
有效和快速的诊断工具对于在医疗机构及时治疗和控制CRGNB的感染至关重要.

研究的目的:

评估和比较用于检测CRGNB的传统和分子诊断方法.
评估染色基质 (HiCrome KPC Agar) 与其他方法 (如MacConkey盘和VITEK 2) 进行CRGNB识别的疗效.

主要方法:

从重症监护室 (ICU) 的患者样本和医院环境中收集了94个样本.
在染色基质 (HiCrome KPC Agar) 和MacConkey agar上培养,使用伊米佩内姆光盘.
使用VITEK 2自动化系统和16S rRNA基因测序来识别和描述分离物.

主要成果:

与MacConkey磁盘和VITEK-2方法相比,HiCrome KPC agar对CRGNB检测具有很高的敏感性 (99.3%和94.7%).
与VITEK 2和16S rRNA测序相比,HiCrome KPC agar (47.4%的同意) 的细菌识别一致性较低.
在患者和环境样本中,Acinetobacter属被确定为主要的CRGNB.

结论:

HiCrome KPC 是一种敏感且易于使用的CRGNB检测方法,可方便快速诊断和治疗.
乙菌的流行凸显了医疗机构严格的环境清洁和感染控制措施的迫切需要.
该研究提供了关于CRGNB诊断的宝贵数据,并为基因库贡献了细菌菌株 (加入号 OR553657,OR553658,OR553659).

关键词:

16SrRNA 是一个16SrRNA.在CRGNB的基础上.耐卡巴胺细菌 (CRB) 是一种耐卡巴胺的细菌.染色体介质是一种染色体介质.格拉姆阴性细菌 (GNB) 是一种维特克 2 维特克 2

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