环素和链杆菌素联合应用对大米土壤微生物多样性和功能的影响
Sophia Subhadarsini Pradhan1,2, Arabinda Mahanty1, Ansuman Senapati1
1ICAR-National Rice Research Institute, Cuttack, Odisha, 753006, India.
Environmental science and pollution research international
|November 13, 2024
概括
土中的抗生素残留物,如四环素和菌素,对微生物群落产生负面影响. 它们的应用减少了细菌多样性和酶活性,联合抗生素造成了最严重的伤害.
科学领域:
- 环境科学 环境科学
- 微生物学 微生物学
- 土壤科学 土壤科学
背景情况:
- 在农业中使用抗生素导致土壤污染.
- 了解抗生素残留的持久性和生态影响至关重要.
研究的目的:
- 在大米土壤中量化抗生素残留物 (四环素,链杆菌素,链杆菌素).
- 评估这些抗生素对土壤微生物社区结构和功能的影响.
主要方法:
- 微观世界实验不同剂量的抗生素 (0.5RD,RD,2RD).
- 使用衰变模型和半衰期确定 (DT50) 量化残留物.
- 基因组分析 (Illumina-MiSeq) 用于评估微生物群落结构和多样性指数 (Shannon,Simpson,ACE,Chao1).
- 测量土壤酶活动 (脱酶,β-葡萄糖酶等). ) 的情况.
主要成果:
- 在RD时,四环素在9天内降解;菌素和环素需要21天.
- 环素的半衰期为46.5-53.3小时,链菌素为177.6-198小时.
- 抗生素的应用显著降低了土壤酶的活动.
- 甲基因组分析显示,主要细菌类 (Chloroflexi,Actinobacteria等) 的抑制. ) 的情况.
- 细菌的多样性下降,而链状环素显示出最突出的减少.
结论:
- 抗生素残留物存在于大米土壤中,降解速度各不相同.
- 抗生素会对土壤微生物社区的结构,多样性和功能产生负面影响.
- 联合抗生素 (链环素) 的负面影响比单独的抗生素更为明显.
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