相关实验视频

Updated: Jun 7, 2025

Visualizing Protein Kinase A Activity In Head-fixed Behaving Mice Using In Vivo Two-photon Fluorescence Lifetime Imaging Microscopy

Visualizing Protein Kinase A Activity In Head-fixed Behaving Mice Using In Vivo Two-photon Fluorescence Lifetime Imaging Microscopy

Published on: June 7, 2019

通过定量成像揭示托芬在模型生物中的动态和功能

Kui Wang¹, Tian-Lun Chen¹, Xin-Xin Zhang^1,2

¹Institute of Neuroscience, State Key Laboratory of Neuroscience, Center for Excellence in Brain Science and Intelligence Technology, Chinese Academy of Sciences, 320 Yue-Yang Road, Shanghai, 200031, China.

|November 13, 2024

概括

一个新的绿色酸盐比度指标 (GRIT) 允许精确测量酸盐动态. 该工具揭示了免疫反应,神经递质水平和行为之间的联系,有助于健康和疾病研究.

科学领域:

生物化学生物化学
神经科学是一个神经科学.
免疫学免疫学免疫学

背景情况:

托芬是一种必不可少的氨基酸,对色胺和色胺的产生至关重要,影响神经和免疫功能.
准确的托测量对于理解这些复杂的生物过程至关重要.

研究的目的:

开发和验证一种用于定量测量托的新方法.
研究托芬在各种生物尺度上的动态,从细胞到整个生物体.

主要方法:

为托 (GRIT) 开发一个绿色比值指标.
在细菌,哺乳动物细胞培养 (线粒体),人体血清和斑马鱼中应用GRIT.
在各种生物系统中对托芬动态的定量分析.

主要成果:

GRIT成功量化了细菌,细胞培养,血清和斑马鱼中的托芬动态.
细胞规模分析显示了细胞类型和细胞器上不同的托芬动态.
在斑马鱼中进行的全身研究表明,炎症诱导的酸盐增加与血清激素/肌氨酸升高,睡眠延长有关,并表明免疫力和行为之间存在代谢联系.
在患有炎症的患者中,GRIT检测到血清酸盐降低,这表明了诊断潜力.

结论:

格里特是研究托代谢及其生理作用的强大工具.
这种方法为健康和疾病的代谢调节提供了新的见解.
格里特促进了跨多个生物尺度的研究,从细胞过程到生物行为的研究.

关键词:

细菌细菌是一种细菌.人类血清的人类血清线粒体中的线粒体.三丹定量成像成像技术斑马鱼是一种斑马鱼.

更多相关视频

Radiosynthesis of 1-2-[18F]Fluoroethyl-L-Tryptophan using a One-pot, Two-step Protocol

Radiosynthesis of 1-2-[18F]Fluoroethyl-L-Tryptophan using a One-pot, Two-step Protocol

Published on: September 21, 2021

In vivo Quantification of G Protein Coupled Receptor Interactions using Spectrally Resolved Two-photon Microscopy

In vivo Quantification of G Protein Coupled Receptor Interactions using Spectrally Resolved Two-photon Microscopy

Published on: January 19, 2011

相关概念视频

Protein Dynamics in Living Cells

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...