基于H-NMR对茄子或胡果在发育过程中的H-NMR分析的代谢物量化数据
Léa Roch1, Catherine Deborde1,2,3,4, Daniel Jacob1,2,3,4
1INRAE, Univ. Bordeaux, Biologie du Fruit et Pathologie, UMR 1332, Centre INRAE de Nouvelle Aquitaine, Bordeaux, 33140, Villenave d'Ornon, France.
BMC research notes
|November 14, 2024
概括
这项研究量化了胡和茄子水果在发育过程中的主要极地代谢物. 这些数据支持对水果新陈代谢调节的研究和比较的奥米克学研究.
科学领域:
- 植物代谢学 植物代谢学
- 果物发育生物学 果物发育生物学
- 索兰氏植物作物研究
背景情况:
- 水果的主要代谢物积累在发育过程中受到复杂的调节.
- 番茄 (Solanum lycopersicum) 是Solanaceae水果代谢研究的一个模型.
- 胡 (Capsicum annuum L.) 和茄子 (Solanum melongena L.) 提供了有价值的比较见解.
研究的目的:
- 提供胡和茄子果实中主要极地代谢物的定量数据.
- 为了使各种水果物种的比较代谢学研究.
- 促进与其他omics数据的整合,以获得更深入的监管见解.
主要方法:
- 在10-11个发育阶段对胡和茄子果实进行量化代谢物分析.
- 极地提取物的质子核磁共振 (1H-NMR) 代谢分析.
- 对于胡中的24种代谢物和茄子中的27种代谢物,数据表达为每公克新鲜体重的μmol.
主要成果:
- 量化了胡和茄子之间19种常见的代谢物,包括糖,有机酸和氨基酸.
- 对于这些作物,主要的极地代谢物的详细发育概况现在已可用.
- 建立了一个定量数据集,用于跨物种和跨物种的比较.
结论:
- 生成的数据集是了解Solanaceae中的水果代谢调节的宝贵资源.
- 这些数据支持比较分析和整合多学科信息.
- 有助于识别果实发育过程中保存和特定物种的代谢途径.
相关概念视频
01:07Proton (¹H) NMR: Chemical Shift
Organic molecules primarily contain carbon and hydrogen atoms. While all the hydrogen isotopes are NMR-active, protium or hydrogen-1 is the most abundant. It has a significant energy separation between its nuclear spin states due to its large gyromagnetic ratio. As per Boltzmann's distribution, an increase in the energy separation implies a greater excess population of nuclei available for excitation, resulting in a strong NMR absorption signal.
Absorption signals of all the protium nuclei in a...
Absorption signals of all the protium nuclei in a...
01:13¹H NMR: Complex Splitting
A proton M that is coupled to a proton X results in doublet signals for M. However, NMR-active nuclei can be simultaneously coupled to more than one nonequivalent nucleus. When M is coupled to a second proton A, such as in styrene oxide, each peak in the doublet is split into another doublet.
Splitting diagrams or splitting tree diagrams are routinely used to depict such complex couplings. While drawing splitting diagrams, the splitting with the larger coupling constant is usually applied first.
Splitting diagrams or splitting tree diagrams are routinely used to depict such complex couplings. While drawing splitting diagrams, the splitting with the larger coupling constant is usually applied first.
00:48¹H NMR of Labile Protons: Deuterium (²H) Substitution
This lesson illustrates the role of deuterium substitution in simplifying the NMR spectrum of compounds comprising labile protons. One method employed is the use of deuterium. Amongst the three isotopes of hydrogen, deuterium (2H) has a nucleus composed of one proton and one neutron. When the D2O solvent is added to a pure dry ethanol solution, its labile proton is substituted with deuterium.
00:58¹H NMR Signal Integration: Overview
The intensity of a signal, which can be represented by the area under the peak, depends on the number of protons contributing to that signal. The area under each peak is shown as a vertical line called an integral, with the integral value listed under it, as seen in the proton NMR spectrum of benzyl acetate. Each integral value is divided by the smallest integral value to obtain the ratio of the number of protons producing each signal. The ratio reveals the relative number of protons and not...
01:09¹H NMR: Pople Notation
The Pople nomenclature system classifies spin systems based on the difference between their chemical shifts. Coupled spins are denoted by capital letters with subscripts indicating the number of equivalent nuclei. When the coupled nuclei have well-separated chemical shifts, they are assigned letters that are far apart in the alphabet, such as A and X. When the difference in chemical shifts is small, coupled nuclei are named using adjacent letters of the alphabet (AB, MN, or XY).
A proton...
A proton...
01:15¹H NMR of Conformationally Flexible Molecules: Variable-Temperature NMR
The axial and equatorial protons in cyclohexane can be distinguished by performing a variable-temperature NMR experiment. In this process, except for one proton, the remaining eleven protons are replaced by deuterium. The deuterium substitution avoids the possible peak splitting caused by the spin-spin coupling between the adjacent protons. The remaining proton flips between the axial and equatorial positions.