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Confocal Fluorescence Microscopy

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...

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相关实验视频

Updated: Jun 7, 2025

Spectral Reflectometric Microscopy on Myelinated Axons In Situ

Spectral Reflectometric Microscopy on Myelinated Axons In Situ

Published on: July 2, 2018

使用轴记忆效应进行深度分辨率斑点相关成像.

Shaurya Aarav, Jason W Fleischer

|November 14, 2024

概括

此摘要是机器生成的。

斑点相关成像现在通过从两个距离捕获图像来恢复对象深度. 这种非侵入性技术扩展了透过雾或生物组织等散射介质的视觉.

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相关实验视频

Last Updated: Jun 7, 2025

Spectral Reflectometric Microscopy on Myelinated Axons In Situ

Spectral Reflectometric Microscopy on Myelinated Axons In Situ

Published on: July 2, 2018

Highly Resolved Intravital Striped-illumination Microscopy of Germinal Centers

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Published on: April 9, 2014

Using Digital Image Correlation to Characterize Local Strains on Vascular Tissue Specimens

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Published on: January 24, 2016

科学领域:

光学和光子学在光学和光子学.
生物医学成像学生物医学成像学

背景情况:

斑点相关成像 (SCI) 是一种用于恢复被散射介质遮蔽的2D对象的角大小的技术.
传统的SCI的一个主要局限性是它无法确定物体的深度.

研究的目的:

扩展斑点相关成像 (SCI) 以实现对象深度的非侵入性恢复.
开发一种不需要访问散射介质对象侧的方法.

主要方法:

在两个不同的探测器距离捕获对象的斑点图像.
利用轴记忆效应的缩放特性来确定深度.

主要成果:

成功扩展SCI以恢复对象深度.
通过散射介质展示了一种通过散射介质进行深度恢复的非侵入性方法.

结论:

增强的SCI方法为非侵入性深度测量提供了新的能力.
这种技术在生物成像中具有潜在的应用,可以克服雾或其他散射环境引起的可见性限制.