促炎性细胞因子对尿路细胞腺三酸盐释放和分解的影响
Belinda Kaleska1, Ronald Sluyter2, Zhuoran Chen3
1Graduate School of Medicine, Faculty of Science, Medicine and Health, University of Wollongong, Wollongong, NSW 2522, Australia.
Bladder (San Francisco, Calif.)
|November 14, 2024
概括
尿液中的促炎性细胞因子在患有脱子过活性 (DO) 和尿路感染 (UTI) 的女性中升高. 这些细胞因子影响膀尿细胞中的腺三酸盐 (ATP) 信号传递,影响释放和分解.
科学领域:
- 泌尿器科 泌尿器科 泌尿器科 泌尿器科
- 细胞生物学 细胞生物学
- 免疫学 免疫学 免疫学
背景情况:
- 尿路症状如急性和频率与膀炎有关,但确切的原因尚不清楚.
- 通过纯能受体传递细胞外腺三酸盐 (ATP) 信号是膀感觉的已知途径.
- 促炎性细胞因子在改变膀病理中的ATP信号传递中的作用需要进一步研究.
研究的目的:
- 为了与健康对照对比,在患有尿管感染 (UTI) 或不患有尿管感染 (DO) 的女性中,比较尿中促炎性细胞因子水平.
- 为了研究关键的促炎细胞因子对腺三酸盐 (ATP) 释放和分解在人类尿细胞的影响.
主要方法:
- 干扰素- (IFN-γ),瘤亡因子-阿尔法 (TNF-α) 和介素-1β (IL-1β) 的尿度在患有DO (有/没有尿路感染) 和对照女性中被测量.
- 人类泌尿管细胞 (UROtsa) 已先用单个或组合的细胞因子 (IFN-γ,TNF-α,IL-1β) 进行预处理.
- 随后分析了对低血压诱导的ATP释放和核酸分解的影响.
主要成果:
- 在患有DO和并发性尿路感染的妇女中观察到IFN-γ,TNF-α和IL-1β尿液水平升高.
- 单独的细胞因子减少了ATP的释放,而所有三种细胞因子的组合诱导了低血压诱导的ATP释放的适度增加.
- 细胞因子预处理显著增强了尿细胞对核酸的分解.
结论:
- 尿对促炎性细胞因子的反应是复杂的,影响ATP的释放和分解.
- 这些发现表明,在DO和UTI的病理生理学中,ATP信号的细胞因子介导的改变涉及潜在的机制.
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