花发酵溶液的制备及其生物活性研究
Nan Tang1, Xiaoqing Xu2, Zhenyu Guo1
1School of Base Medical Sciences, Guangdong Pharmaceutical University, Guangzhou, China.
Frontiers in microbiology
|November 14, 2024
概括
酵母发酵增强了三花的抗氧化和抗炎性质. 由此产生的松花发酵溶液是安全的,适合作为天然化品成分使用.
科学领域:
- 生物技术是生物技术.
- 药理学 药理学是指药理学的学科.
- 化品科学 化品科学
背景情况:
- 红杉是一种传统的中医药,具有宝贵的抗氧化和抗炎性质.
- 它的化学成分,包括黄类,多糖和类,有助于其药理作用.
- 由于这些特性,红杉因其在化品行业的潜力而越来越受认可.
研究的目的:
- 为了优化叶绿花的酵母发酵条件.
- 评估发酵红杉的增强抗氧化和抗炎功效.
- 评估花发酵溶液用于化品应用的安全性.
主要方法:
- 用单因素实验和响应表面方法来确定使用酵母菌株YF-5的最佳发酵条件.
- 在细胞和斑马鱼模型中,通过测量甲基 (MDA) 和超氧化物脱酶 (SOD) 活性以及总抗氧化能力 (T-AOC) 来评估抗氧化活性.
- 通过在细胞模型中测量TNF-α和NO抑制,并通过在斑马鱼模型中比较抗炎活性与德克萨来评估抗炎作用.
- 使用胚胎胆膜 (CAM) 试验评估了化品的安全性.
主要成果:
- 最佳发酵参数包括36.55°C的温度,材料与液体的比例为1:20.46,6.20%的果糖,72小时的持续时间和4%的注射剂.
- 发酵显著增强了红杉的抗氧化和抗炎生物活性.
- 松花发酵溶液抑制了MDA的产生,增加了SOD活性和T-AOC.
- 它显示了TNF-α和NO释放的显著抑制,在斑马鱼模型中具有与德克萨米他相当的抗炎活性.
- 在CAM测试表明没有显著的损害,表明良好的安全性.
结论:
- 酵母发酵有效地增强了红杉的抗氧化和抗炎性质.
- 松花发酵溶液表现出强大的生物活性,并通过了化品安全评估.
- 它代表了一种有前途的新天然成分,用于化品产品配方.
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