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相关概念视频

Two-dimensional Gel Electrophoresis01:22

Two-dimensional Gel Electrophoresis

5.8K
Two-dimensional gel electrophoresis is a high-resolution protein separation method first introduced by O' Farrell and Klose in 1975. This method involves protein separation by two dimensions, mass and charge, making it more accurate than one-dimensional gel electrophoresis.
The first dimension separation uses the isoelectric focusing or IEF technique performed on immobilized pH gradient (IPG) strips that separate proteins according to their isoelectric points.
Biological samples, such...
5.8K
SDS-PAGE01:27

SDS-PAGE

27.4K
Gel electrophoresis is a method that separates biological macromolecules like nucleic acids or proteins by forcing them to pass through a gel matrix under an electric field.
A variation of gel electrophoresis, termed  polyacrylamide gel electrophoresis (PAGE), is commonly used for separating proteins according to their molecular size by passing them through a polyacrylamide gel. Because of the varying charges associated with amino acid side chains, PAGE can be used to separate intact...
27.4K
DNA Agarose Gel Electrophoresis02:35

DNA Agarose Gel Electrophoresis

96.0K
Agarose gel electrophoresis is a laboratory technique commonly used to separate DNA fragments by size. However, it can also be used to isolate and purify DNA fragments using a gel extraction protocol.
Gel extraction follows five major steps: running gel electrophoresis to separate fragments, isolating the individual bands, extracting DNA from those bands, and removing the dye and salts from the extracted mixture to obtain pure DNA.
In cloning experiments, both the insert and vector DNA...
96.0K

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相关实验视频

Updated: Jun 7, 2025

Light-mediated Formation and Patterning of Hydrogels for Cell Culture Applications
10:45

Light-mediated Formation and Patterning of Hydrogels for Cell Culture Applications

Published on: September 29, 2016

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堵塞的皮克林格乳液凝

Jia Zhang1,2, Yuan Zheng2, Baoling Guo3

  • 1Department of Medical Oncology, The First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang, 310003, P. R. China.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)
|November 14, 2024
PubMed
概括
此摘要是机器生成的。

研究人员使用纳米粒子和PEG分子开发了Jammed Pickering乳液凝 (JPEGs). 这些新型乳液凝为各种应用提供了增强的稳定性和生物相容性.

关键词:
皮克林乳液是一种皮克林乳液.合性表面活性剂是一种合性表面活性剂.药物输送是药物输送的过程.乳液凝是一种乳液凝.接口工程 接口工程

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Characteristics of Precipitation-formed Polyethylene Glycol Microgels Are Controlled by Molecular Weight of Reactants
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Characteristics of Precipitation-formed Polyethylene Glycol Microgels Are Controlled by Molecular Weight of Reactants

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Traction Microscopy Integrated with Microfluidics for Chemotactic Collective Migration
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Traction Microscopy Integrated with Microfluidics for Chemotactic Collective Migration

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相关实验视频

Last Updated: Jun 7, 2025

Light-mediated Formation and Patterning of Hydrogels for Cell Culture Applications
10:45

Light-mediated Formation and Patterning of Hydrogels for Cell Culture Applications

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Characteristics of Precipitation-formed Polyethylene Glycol Microgels Are Controlled by Molecular Weight of Reactants
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Characteristics of Precipitation-formed Polyethylene Glycol Microgels Are Controlled by Molecular Weight of Reactants

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Traction Microscopy Integrated with Microfluidics for Chemotactic Collective Migration
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Traction Microscopy Integrated with Microfluidics for Chemotactic Collective Migration

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科学领域:

  • 材料科学 材料科学 材料科学
  • 合体和表面化学
  • 类风病学 类风病学 类风病学

背景情况:

  • 乳液凝在食品,化品和生物医学行业至关重要,但由于油相相互作用有限,制造油中的水乳液凝仍然具有挑战性.
  • 稳定油中的水乳液通常需要有效的界面稳定机制.

研究的目的:

  • 开发一种用于构建稳定的油中的水乳液凝的多功能策略.
  • 引入使用纳米粒子和特定交联分子的Jammed Pickering乳液凝 (JPEG).

主要方法:

  • 稳定油中的水皮克林乳液使用SiO2纳米颗粒作为油阶段的合表面活性剂.
  • 在水/油界面的负电荷SiO2纳米粒子与水相中的正电荷NH2-PEG-NH2分子进行交叉链接.
  • 诱导凝通过通过接口交叉连接堵塞乳液系统,防止滴滴变形.

主要成果:

  • 成功制备适用于各种油阶段的凝结皮克林乳液凝 (JPEG).
  • JPEG显示出出色的生物相容性,剪切稀释性能,并在广泛的温度范围内和离心过程中保持稳定性.
  • 在肠道条件下表现出对湿组织的良好粘附性和受控的持续释放.

结论:

  • 开发的策略为创建具有理想性质的新型乳液凝提供了一种多功能方法.
  • JPEGs代表了一个有前途的交付平台,在组织工程和控制释放系统中具有潜在的应用.
  • 预计这种方法将激发基于乳液的系统材料设计的进一步创新.