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有机废物和木纤维素混合物糖化和通过发酵对水解盐的验证
Stanislav Rudnyckyj1, Sergey Kucheryavskiy2, Tanmay Chaturvedi3
1Department of Energy, Aalborg University, Niels Bohrs Vej 8, 6700, Esbjerg, Denmark. stru@energy.aau.dk.
Applied microbiology and biotechnology
|November 14, 2024
概括
这项研究表明,将城市固体废物的有机分量 (OFMSW) 添加到木纤维素中,可以改善发酵介质. OFMSW作为合成剂的可持续替代品,促进酵母菌的生长和生物炼油厂的乙醇生产.
科学领域:
- 生物技术和生物工程 生物技术和生物工程
- 可持续材料科学科学 可持续材料科学
- 可再生能源可再生能源是可再生能源.
背景情况:
- 开发可持续的发酵介质对于纤维素生物炼油厂至关重要.
- 城市固体废物 (OFMSW) 是一个潜在的低成本,丰富的资源.
- 有机溶解的木纤维素需要高效的酶性水解来生产糖.
研究的目的:
- 调查OFMSW集成对有机溶解的木纤维素的酶性水解的影响.
- 评估OFMSW作为发酵介质中合成剂的可持续替代品的潜力.
- 评估使用OFMSW-纤维素混合物的乙醇和细胞生物质的生产.
主要方法:
- 在不同比例下将OFMSW与有机溶解的木纤维素混合.
- 使用Cellic® CTec3在不同的酶剂量下进行酶性水解.
- 用Saccharomyces cerevisiae和Mucor indicus发酵生成的水解盐,用于乙醇和生物质生产.
主要成果:
- 包括OFMSW (35-45%) 的糖含量与纯纤维素 (58-68%的糖化) 相当.
- OFMSW-纤维素混合物支持S. cerevisiae生物质的产量是纤维素水解与合成的三倍.
- 乙醇产量达到30g/L;然而,M. indicus的生长被抑制,可能是OFMSW化合物.
结论:
- OFMSW是发酵介质的成本效益和可持续成分,作为缓冲和源.
- OFMSW增强了S. cerevisiae的生长,并减少了生物炼油厂对合成剂的依赖.
- OFMSW的抑制化合物需要进一步调查,以优化其与M. indicus.等特定微生物的使用.
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