基于I-Motif DNA的光定量微针传感贴片,用于对间歇性液体中微小的pH值变化产生敏感反应
Lin He1, Yan Zhou1, Min Zhang1
1State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, College of Biology, Key Laboratory for Bio-Nanotechnology and Molecular Engineering of Hunan Province, Hunan University, Changsha 410082, China.
ACS sensors
|November 14, 2024
概括
一个新的微针感应贴片使用i-motif DNA来通过皮肤检测细微的皮肤pH值变化. 这种微创技术为健康和疾病状态提供了高分辨率的监控.
科学领域:
- 生物医学工程 生物医学工程
- 材料科学 材料科学 材料科学
- 分析化学 分析化学
背景情况:
- 检测皮肤间歇液 (ISF) 中的轻微pH值变化对于了解健康和疾病至关重要.
- 目前用于ISF pH监测的方法具有挑战性和侵入性.
研究的目的:
- 开发一种微创,高分辨率和敏感的皮肤间监测策略,用于ISF中微小的pH变化.
- 创建一个基于i-motif DNA的光比度微针传感贴片 (IFR-pH MN贴片).
主要方法:
- 通过交叉链接凝甲基和甲基化氨酸制造了一个微针贴片.
- 在补丁矩阵内集成的pH敏感头发针含有i-动机DNA的光比度探针.
- 评估ISF提取量和时间,模仿皮肤模型中的pH量测准确度,以及小鼠模型中的体内表现.
主要成果:
- IFR-pH MN贴片表现出强大的皮肤透和高胀能力,提取足够的液体 (在10分钟内≤56.4μL).
- 该贴片准确量化了6.2-6.9范围内的pH值,在模仿皮肤模型中准确度为0.2pH单位.
- 在伤口和瘤小鼠模型上的体内测试证实了该贴片能够获得透皮pH值的能力.
结论:
- 开发的IFR-pH MN贴片可实现最小侵入性,敏感性和高分辨率的透皮pH监测.
- 生物相容贴片显示了通过追踪皮肤间pH值来监测和干预病态的潜力.
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