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相关概念视频

Experimental RNAi02:15

Experimental RNAi

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RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
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siRNA - Small Interfering RNAs02:30

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Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the...
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Small interfering RNAs (siRNA)02:30

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RNA Interference01:23

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RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
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相关实验视频

Updated: Jun 7, 2025

MISSION esiRNA for RNAi Screening in Mammalian Cells
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siRNAEfficacyDB:一个经过实验支持的小干扰RNA疗效数据库.

Yang Zhang1, Ting Yang2, Yu Yang2

  • 1Innovative Institute of Chinese Medicine and Pharmacy, Academy for Interdiscipline, Chengdu University of Traditional Chinese Medicine, Chengdu, China.

IET systems biology
|November 14, 2024
PubMed
概括

一个新的数据库,siRNAEfficacyDB,为3544个记录提供了关于小干扰RNA (siRNA) 疗效的验证数据. 该资源有助于优化siRNA药物设计和推进核酸疗法.

关键词:
这是一个RNARNARNARNARNA.大数据就是大数据.药物 药物 药物 药物 药物 药物

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科学领域:

  • 生物技术是生物技术.
  • 分子生物学分子生物学
  • 生物信息学是一种生物信息学.

背景情况:

  • 小干扰RNA (siRNA) 技术可以为生物医学研究和药物开发提供精确的基因沉默.
  • 在siRNA疗法当前的挑战包括交付效率,目标特异性和分子稳定性.
  • 克服这些障碍对于推进基于siRNA的治疗方法至关重要.

研究的目的:

  • 开发siRNAEfficacyDB,一个全面的实验验证的siRNA疗效数据数据库.
  • 为参与siRNA药物设计和开发的研究人员提供集中资源.
  • 为了促进计算机辅助siRNA设计和核酸药物发现.

主要方法:

  • 来自实验研究的3544个siRNA记录的汇编.
  • 数据的整合,包括siRNA序列,目标基因,抑制效率和实验条件.
  • 开发一个用户友好的Web界面,用于数据查询,浏览和分析.

主要成果:

  • 该数据库涵盖了42个目标基因和5个细胞系.
  • 包括关于siRNA序列,基因,抑制效率,实验技术,细胞系,度和潜伏时间的详细信息.
  • 网页界面允许高效的数据访问和分析.

结论:

  • siRNAEfficacyDB作为siRNA药物设计和优化的一个有价值的数据基础.
  • 该数据库支持计算机辅助siRNA设计研究的进展.
  • 它是开发基于核酸的药物的关键资源.