一个闭环的模块化多器官芯片平台,用于自我维持和严格控制的氧化
Nan Jiang1,2, Guoliang Ying1, Yixia Yin1
1Division of Biomedical Engineering, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02139.
概括
这项研究引入了一种新的多器官芯片平台,用于在微环境中精确控制氧气. 该系统能够实现生理上相关的氧气水平,改善器官芯片模型中的药物代谢研究.
科学领域:
- 生物技术是生物技术.
- 物理工程生理学工程
- 药物发现 药物发现 药物发现
背景情况:
- 生理微环境对于器官芯片 (OOC) 系统来准确评估药物代谢至关重要.
- 目前的OOC系统通常使用环境化器氧气水平 (21%),与组织特异度 (0.5-13%) 相比,在生理上无关紧要.
- 氧气水平显著影响细胞和组织功能以及药物代谢.
研究的目的:
- 开发一个闭环模块化多器官芯片平台,用于实时监测和精确控制氧气水平.
- 通过连接的微组织,使溶解氧在4-20%的范围内进行独立调整.
- 在OOC模型中研究受控氧微环境对药物代谢的影响.
主要方法:
- 开发一个模块化的多器官芯片平台,集成微流体氧吸收器,氧气发生器和监控/控制系统.
- 使用生物反应器进行连接微组织的循环培养.
- 在受控的氧气条件下对平行连接的肝脏,脏和动脉血管微组织进行药物研究.
主要成果:
- 该平台成功实现了实时监测和严格控制培养基中氧气水平 (4-20%).
- 证明氧气水平显著影响肝脏,脏和动脉血管微组织中的药物代谢.
- 展示了平台提供生理相关和独立调节的氧气微环境的能力.
结论:
- 开发的平台通过提供受控制的氧微环境来增强OOC系统的生理相关性.
- 这项技术可以提高药物查和代谢研究的准确性和性能.
- 该平台支持单个和多个器官在芯片上的配置,以满足各种研究需求.
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