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在LED蓝光照射下,ROS/AKT/S6K轴诱导角膜上皮功能障碍

Le Jin¹, Qianjie Yang¹, Jiafeng Li²

¹Department of Ophthalmology, the First Affiliated Hospital of Zhejiang University, Hangzhou, Zhejiang, China.

Ecotoxicology and environmental safety

|November 14, 2024

概括

来自LED的蓝光 (BL) 通过增加氧化应激和亡来损害角膜细胞. 针对ROS/PI3K/AKT/S6K通路进行NAC和SC79等治疗,可以防止这种损伤.

科学领域:

眼科医生眼科眼科
细胞生物学细胞生物学
毒理学毒理学毒理学

背景情况:

发光二极管 (LED) 发出高能蓝光 (BL),引起了人们对潜在的眼睛健康问题的担忧.
角膜上皮功能障碍 (CEpD) 是BL暴露的潜在后果,但根本机制尚未完全理解.

研究的目的:

阐明BL暴露诱导CEpD的特定分子机制.
研究活性氧物种 (ROS) 和PI3K/AKT通路在BL诱导的角膜损伤中的作用.

主要方法:

人类角膜上皮细胞 (hCEpC) 暴露于440nm BL.
评估细胞迁移,ROS生产和细胞亡.
RNA测序和生物信息分析.
在细胞和小鼠模型中用N-乙半氨酸 (NAC) 和SC79 (SC) 治疗.
抑制和激活AKT/S6K路径.

主要成果:

BL暴露减少了hCEpC的迁移,增加了ROS的产生和亡.
RNA-Seq揭示了对细胞氧化还原,伤口愈合,亡调节以及PI3K/AKT通路的显著影响.
NAC治疗恢复了细胞活力,AKT/S6K激活,并逆转了BL诱导的亡和迁移.
在小鼠中,AKT抑制加剧了BL诱导的损伤,而SC和NAC治疗提供了保护.

结论:

通过ROS/PI3K/AKT/S6K信号级联,BL暴露会诱导CEpD.
准这种途径为缓解BL诱导的眼损伤提供了潜在的治疗策略.
这些发现为保护角膜免受过度BL暴露提供了洞察力.

关键词:

蓝光是蓝色的光,蓝光是蓝光的.角膜上皮功能障碍角膜上皮功能障碍发光二极管 (LED) 是一种发光二极管.在PI3K/AKT信号系统中.反应性氧物种 (ROS) 是一种反应性氧物种.

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