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Updated: Jun 7, 2025
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Diagnosing Pulmonary Tuberculosis with the Xpert MTB/RIF Test
Published on: April 9, 2012
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为Xpert MTB/RIF Ultra测定汇集唾液样本:一种敏感且有效的查策略
Zhuo Quan1, Yong Qiu2, Meng Li1
1Shanghai Institute of Infectious Disease and Biosecurity, Key Laboratory of Medical Molecular Virology (MOE/ NHC/CAMS), School of Basic Medical Science, Fudan University, Shanghai, China.
Tuberculosis (Edinburgh, Scotland)
|November 14, 2024
概括
使用Xpert MTB/RIF Ultra (Ultra) 的集体测试显示了对结核病 (TB) 查的高灵敏度. 这种方法可以节省大量的弹药盒,特别是在8个池的大小的情况下,这使得它对大规模结核病检测有效.
科学领域:
- 微生物学 微生物学
- 传染性疾病 传染性疾病
- 分子诊断学 分子诊断
背景情况:
- 标准的Xpert MTB/RIF测定在诊断乳结核病时具有有限的灵敏度.
- 正在探索聚合测试策略,以提高结核病诊断的效率和成本效益.
研究的目的:
- 评估Xpert MTB/RIF Ultra (Ultra) 测试用于大规模结核病查的综合测试的敏感性和有效性.
- 为了确定各种结核病流行率的Ultra聚合测试的最佳池大小.
主要方法:
- 以前通过个别的Xpert测试进行测试的冷唾液样本被分成4,8和16组.
- 采用Xpert MTB/RIF Ultra试验测试了组合样本,每个组合包含一个不同细菌负载的单个阳性样本.
- 对结核病查场景的模拟在0.2-1.0%的流行范围进行,以计算弹药盒的效率.
主要成果:
- 超级聚合测试的总体灵敏度很高,报告为88.9% (95% CI:75.9-96.3).
- 当阳性样本具有更大的真菌细菌负载时,灵敏度更高.
- 一个8个池的大小显示至少80%的弹药盒节省,即使是在最高的模拟流行率.
结论:
- 使用Xpert MTB/RIF Ultra的唾液聚合是一种敏感且有效的结核查策略.
- 尽管它是有效的,但在资源有限的环境中广泛的查可能需要开发新的,成本较低的,高通量工具,可能利用非样.
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