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基于生物信息学识别和验证胰腺β细胞衰老过程中关键的调控转录因子YY1的有效性

Junqi Yang¹, Yumin Ma², Jiang Liu¹

¹Pancreatic Center, Department of Gastroenterology, Affiliated Hospital of Yangzhou University, Yangzhou University, Yangzhou, Jiangsu, China; Yangzhou Key Laboratory of Pancreatic Disease, The Affiliated Hospital of Yangzhou University, Yangzhou University, Yangzhou, Jiangsu, China.

Experimental gerontology

|November 14, 2024

概括

胰腺β细胞衰老会影响葡萄糖耐受性. 这项研究确定了YY1转录因子作为一个关键的调节器,其激素eudesmin (EDN) 通过抑制P38/JNK MAPK通路来改善葡萄糖不耐受性和β细胞衰老.

科学领域:

细胞和分子生物学细胞和分子生物学
内分泌学在内分泌学.
老年学是指老年学的学科.

背景情况:

胰腺β细胞衰老与葡萄糖耐受性受损和糖尿病有关.
调节胰腺β细胞衰老的精确分子机制尚未完全理解.
确定关键的调节因素对于开发治疗策略至关重要.

研究的目的:

确定参与胰腺β细胞衰老的关键调节因素.
调查针对这些因素对与年龄相关的葡萄糖不耐症的治疗潜力.

主要方法:

来自年轻和老鼠胰腺β细胞的高通量测序数据的生物信息分析.
使用D-银糖诱导的老化小鼠模型和H2O2诱导的MIN6细胞老化模型.
评估了YY1主激素eudesmin (EDN) 对葡萄糖不耐受,β细胞衰老,P21表达和MAPK信号通路 (P38/JNK) 的影响.

主要成果:

确定了转录因子阴阳1 (YY1) 作为胰腺小岛衰老的关键调节者.
在衰老的胰腺β细胞模型中观察到YY1表达的减少.
欧德斯 (EDN) 治疗改善了葡萄糖耐受性,缓解了β细胞衰老,降低了P21的调节,并抑制了P38/JNK MAPK通路.

结论:

证实了YY1转录因子在胰腺β细胞衰老中的调节作用.
欧德斯 (EDN) 证明了胰腺老化相关疾病的治疗潜力.
研究结果表明,针对YY1和相关途径作为治疗葡萄糖耐受性损害和糖尿病的新型临床方法.

关键词:

衰老的衰老衰老的衰老生物信息学是一种生物信息学.在P38/JNK MAPKK中.胰腺β细胞是胰腺β细胞.YY1 YY1 这是一个很大的问题.

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