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循环RNAcircNFIX调节了肌肉卫星细胞中MEF2C表达的过程,而这种表达发生在性脑患者中

Brigette Romero¹, Parsa Hoque¹, Karyn G Robinson²

¹Department of Medical and Molecular Sciences, University of Delaware, Newark, Delaware, USA.

The Journal of biological chemistry

|November 14, 2024

概括

在脑 (CP) 中减少的循环RNA NFIX (circNFIX) 通过减少MEF2C表达来损害肌肉发育. 这一发现表明circNFIX作为早期CP诊断的潜在生物标志物和治疗点.

科学领域:

分子生物学分子生物学
生物化学生化学
遗传学是一个遗传学.

背景情况:

大脑 (CP) 是一种儿科疾病,其分子机制不明确,阻碍了早期诊断.
循环RNAs (circRNAs) 是新兴的生物标志物,但它们在CP中的作用在很大程度上是未知的.
肌细胞特异增强因子2C (MEF2C) 对于瘤的发展至关重要.

研究的目的:

为了研究circNFIX在脑中的作用.
为了阐明circNFIX对MEF2C表达的调节机制.
探索circNFIX作为CP的潜在生物标志物.

主要方法:

在CP患者肌肉细胞和组织中量化circNFIX和MEF2C表达.
单分子分辨率成像以确定circNFIX亚细胞局部.
生物信息分析和实验验证,以确认circNFIX-miRNA相互作用.
肌细胞分化和融合试验,包括circNFIX敲击实验.

主要成果:

在患有CP的个体的肌肉细胞和骨肌肉组织中,circNFIX显著下调.
circNFIX 作为 miR373-3p 的分子海绵,阻止它抑制 MEF2C 翻译.
减少CP的circNFIX导致MEF2C表达的减少,并随后降低了sarcomere基因的下调.
circNFIX knockdown 损害了肌细胞分化和融合,模仿了 CP 细胞表型.

结论:

通过增加miR373-3p介导的MEF2C抑制,circNFIX的下调有助于CP中瘤发育受损.
circNFIX在维持肌原性能力和瘤完整性方面发挥着至关重要的作用.
circNFIX是一个有前途的治疗标,也是早期诊断脑的潜在生物标志物.

关键词:

在MEF2C中,MEF2C是生物标志物生物标志物脑性麻脑性麻是一种疾病.环形鱼 (circfish) 是一种鱼类.周围的NFIXIX 周围的NFIX 圆形的RNARNA是一个圆形的RNA.在 miR-373-3p 中.这种miRNA的海绵化.监管性的非编码RNAs.卫星细胞卫星细胞卫星细胞性脑性麻性脑性麻

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